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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-6988R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
RuBisCO large subunit
- 抗体名:
核酮糖1,5二磷酸羧化酶/二磷酸核酮糖盐羧化酶大链抗体
- 宿主:
Rabbit
- 适应物种:
Arabidopsis Thaliana, ,Gourd,Lotus,Linden
- 免疫原:
KLH conjugated synthetic peptide derived from Arabidopsis thaliana Rubisco:151-250/475
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-1000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul
| 英文名称 | RuBisCO large subunit |
| 中文名称 | 核酮糖1,5二磷酸羧化酶/二磷酸核酮糖盐羧化酶大链抗体 |
| 别 名 | rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO-large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) [Arabidopsis lyrata]. |
| 规格价格 | 100ul/1980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 植物抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Arabidopsis Thaliana, ,Gourd,Lotus,Linden |
| 产品应用 | WB=1:500-1000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 52kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana Rubisco:151-250/475 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | Function: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Subunit: Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers. Subcellular Location: Plastid, chloroplast. Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover. The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization. Similarity: Belongs to the RuBisCO large chain family. Type I subfamily. SWISS: P00875 Gene ID: 2715621 Database links: Entrez Gene: 2715621 Spinacia oleracea SwissProt: P00875 Spinacia oleracea Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Linden Lysate at 40 ug Primary: Anti-RUBISCO (bs-6988R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD ![]() Sample: Gourd leaf Lysate at 40 ug Lotus leaf Lysate at 40 ug Primary: Anti-RUBISCO (bs-6988R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD |
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文献和实验尔化学奖。(一)C3途径的反应过程C3途径是光合碳代谢中最基本的循环,是所有放氧光合生物所共有的同化CO2的途径。1.过程 由RuBP开始至RuBP再生结束,共有14步反应,均在叶绿体的基质中进行。全过程分为羧化、还原、再生3个阶段。(1)羧化阶段(carboxylation phase) 指进入叶绿体的CO2与受体RuBP结合,并水解产生PGA的反应过程。以固定3分子CO2为例:3RuBP+3CO2+3H2O PGA + 6H+核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)具有双重功能,既能使RuBP
经膜上的运转器(translocator)才能通过;蔗糖、C5`C7糖的二磷酸酯、NADP+、PPi等物质则不能通过。2.基质及内含物 被膜以内的基础物质称为基质(stroma),基质以水为主体,内含多种离子、低分子的有机物,以及多种可溶性蛋白质等。基质是进行碳同化的场所,它含有还原CO2与合成淀粉的全部酶系,其中1,5-二磷酸核酮糖羧化酶/加氧酶(ribulose1,5bisphosphate carboxylase/oxygenase,Rubisco)占基质总蛋白的一半以上。此外,基质中含有氨
|鱼藤酮 Rous sarcoma virus|Rous肉瘤病毒 rubella virus|风疹病毒 rubidomycin|红比霉素 rubisco|核酮糖二磷酸羧化酶-加氧酶 rubredoxin|玉红氧还蛋白[一种不含血红素的铁蛋白] ruffling|[细胞]边缘波动 ruminant|反刍动物;反刍(动物)的 rutin|芸香苷,芦丁
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