FOXN1叉头蛋白N1抗体

FOXN1叉头蛋白N1抗体

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  • ¥780 - 2200
  • LMAI Bio
  • LM-6970R
  • 进口/国产
  • 2025年11月21日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:50-200 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
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    • 详细信息
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-6970R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      FOXN1

    • 抗体名

      叉头蛋白N1抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,

    • 免疫原

      KLH conjugated synthetic peptide derived from human FOXN1:321-420/648

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:50-200 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      50ul  100ul  200ul

    FOXN1叉头蛋白N1抗体
    英文名称 FOXN1
    中文名称 叉头蛋白N1抗体
    别    名 FKHL20; Forkhead box N1; Forkhead box protein N1; FOXN 1; FOXN1; FOXN1_HUMAN; RONU; Rowett nude; Transcription factor winged-helix nude; WHN; Winged helix nude; Winged-helix transcription factor nude.  
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 细胞生物  免疫学  染色质和核信号  转录调节因子  表观遗传学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:50-200 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 69kDa
    细胞定位 细胞核 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human FOXN1:321-420/648 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008].

    Function:
    Transcriptional regulator involved in development.

    Subcellular Location:
    Nucleus.

    Tissue Specificity:
    Expressed in thymus.

    Similarity:
    Contains 1 fork-head DNA-binding domain.

    SWISS:
    O15353

    Gene ID:
    8456

    Database links:

    Entrez Gene: 8456 Human

    Omim: 600838 Human

    SwissProt: O15353 Human

    Unigene: 663679 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 FOXN1叉头蛋白N1抗体
    Sample: Thymus (Rat) Lysate at 40 ug
    Primary: Anti-FOXN1 (bs-6970R) at 1/300 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
    Predicted band size: 69 kD
    Observed band size: 69 kD

    FOXN1叉头蛋白N1抗体
    Paraformaldehyde-fixed, paraffin embedded (Mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FOXN1) Polyclonal Antibody, Unconjugated (bs-6970R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    FOXN1叉头蛋白N1抗体
    Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-FOXN1 Polyclonal Antibody, Unconjugated(bs-6970R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    FOXN1叉头蛋白N1抗体
    Blank control: Hela. 
    Primary Antibody (green line): Rabbit Anti-FOXN1 antibody (bs-6970R)
    Dilution: 1μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody: Goat anti-rabbit IgG-AF647
    Dilution: 1μg /test. 
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    FOXN1叉头蛋白N1抗体
    Blank control (Black line): Hela (Black). 
    Primary Antibody (green line): Rabbit Anti-FOXN1 antibody (bs-6970R) 
    Dilution: 1μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
    Dilution: 1μg /test. 
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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