- ￥4303
- Roche
- 罗氏-11465007001
- 2025年11月04日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
−20°C
- 库存：
需确认
- 保质期：
见产品外包装
- 供应商：
嵘崴达
- 英文名：
Cell Proliferation Kit I (MTT)
- 规格：
2500 TESTS
Cell Proliferation Kit I (MTT)
别名: mtt
产品描述
Features and Benefits
• Safe and easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
• Accurate: The absorbance obtained strongly correlates to the cell number.
• Sensitive: Detect low cell numbers.
• Fast: Process a large number of samples using a multi-well ELISA reader.
The assay is based on the cleavage of the tetrazolium salt MTT in the presence of an electron-coupling reagent. The water-insoluble formazan salt produced must be solubilized in an additional step. Cells grown in a 96-well tissue culture plate are incubated with the MTT solution for approximately 4 hours. After this incubation period, a water-insoluble formazan dye is formed. After solubilization, the formazan dye is quantitated using a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells (see below).
Roche试剂
| 11465007001 | Cell Proliferation Kit I (MTT),细胞增殖试剂盒I |
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| 11814419001 | MINI QUICK SPIN DNA COLUMNS,快速自旋脱氧核糖核酸 |
| 10220647001 | GTP-g-S,鸟苷酸 |
| 11249738001 | Phytohemagglutinin-L (PHA-L),植物血凝素- L |
| 10108146001 | Phosphatase,alkaline (AP),Grad,磷酸酶，碱性磷酸酶（AP） |
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文献和实验MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondrial dehydrogenase enzyme from viable cells to cleave the tetrazolium rings of the pale yellow MTT
The MTT Cell Proliferation Assay
. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means.The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, the reduction
CellTrace CFSE Cell Proliferation Kit
. 实验步骤 For staining cells prior to flow cytometric analysis of cell proliferation or cell division, the protocol below is appropriate. More information on this procedure can be found in reference 7. Our suggested initial conditions
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