CYPA亲环蛋白（亲环素）PPIA抗体

价格¥780 - 2200

品牌LMAI Bio
货号LM-5912R
产地进口/国产
更新日期2025年12月11日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
宿主Rabbit
适应物种Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,

上海联迈生物工程有限公司

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详细信息
技术资料

供应商：
上海联迈生物工程有限公司
库存：
大量
目录编号：
LM-5912R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
1年
抗体英文名：
CYPA
抗体名：
亲环蛋白（亲环素）PPIA抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
免疫原：
KLH conjugated synthetic peptide derived from human Cyclophilin A.:101-165/165
亚型：
IgG
形态：
Lyophilized or Liquid
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
浓度：
1mg/ml
保存条件：
Store at -20 °C
规格：
50ul 100ul 200ul

CYPA亲环蛋白（亲环素）PPIA抗体

英文名称	CYPA
中文名称	亲环蛋白（亲环素）PPIA抗体
别名	Cyclophilin A; CyclophilinA; Cyclosporin A binding protein; Cyclosporin A-binding protein; CYPA; CYPH; Peptidyl prolyl cis trans isomerase A; Peptidyl-prolyl cis-trans isomerase A; Peptidylprolyl isomerase A; PPIA; PPIA protein; PPIA_HUMAN; PPIase A; Rotamase A; RotamaseA; T cell cyclophilin.
	Specific References (1) \| bs-5912R has been referenced in 1 publications. [IF=3.58] Kang, Taewook, et al. "Large-scale analysis of posttranslational modifications in the hippocampus of patients with Alzheimer’s disease using pI shift and label-free quantification without enrichment." Analytical and Bioanalytical Chemistry (2014): 1-14. WB ; Human. PubMed:25120180
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	免疫学信号转导 t-淋巴细胞
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	18kDa
细胞定位	细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Cyclophilin A.:101-165/165
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity (1). Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins (2,3). Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans (4,5). CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain (6). CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication (7,8). Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10). Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Subcellular Location: Cytoplasm. Similarity: Belongs to the cyclophilin-type PPIase family. PPIase A subfamily. Contains 1 PPIase cyclophilin-type domain. SWISS: P62937 Gene ID: 5478 Database links: Entrez Gene: 5478 Human Entrez Gene: 268373 Mouse Entrez Gene: 100360977 Rat Entrez Gene: 100362969 Rat Entrez Gene: 25518 Rat Omim: 123840 Human SwissProt: P62937 Human SwissProt: P17742 Mouse SwissProt: P10111 Rat Unigene: 356331 Human Unigene: 598115 Human Unigene: 5246 Mouse Unigene: 118772 Rat Unigene: 1463 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: Cerebrum (Rat) Lysate at 40 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-CYPA (bs-5912R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 17/18 kD Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-CYPA (bs-5912R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated(bs-5912R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated(bs-5912R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- CYPA antibody(bs-5912R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.