The mean recovery of recombinant Sclerostin (spike: 26 pmol/l and 110 pmol/l) in human samples is: serum (n=5): 93% / 86% EDTA plasma (n=5): 94% / 93% citrate plasma (n=1): 104% / 99%
Dilution linearity:
Expected average dilution of recombinant Sclerostin in human samples for 1+1, 1+3 and 1+7 dilution is:
Expected average dilution of endogenous Sclerostin in human samples for 1+1, 1+3 and 1+7 dilution is: serum (n=7): 100% / 103% / 106% EDTA plasma (n=6): 105% / 108% / 123% citrate plasma (n=2): 91% / 91% / 103%
Cross reactivity: The assay does not detect Noggin. The assay does not detect Wise (SOSTDC1).
Serum values from apparently healthy individuals: Median (serum, n=32): 61.5. pmol/l Median (EDTA plasma, n=24): 87 pmol/l Median (citrate plasma, n=24): 61.5. pmol/l It is recommended to establish the normal range for each laboratory.