- ¥1580
- LMAI Bio
- LM-5590R
- 进口/国产
- 2025年12月18日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Cow, Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-5590R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
phospho-14-3-3 epsilon (Thr232)
- 抗体名：
磷酸化14-3-3E蛋白抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Cow, Sheep,
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human 14-3-3E around the phosphorylation site of Thr232:LW(p-T)SD
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul
| 英文名称 | phospho-14-3-3 epsilon (Thr232) |
| 中文名称 | 磷酸化14-3-3E蛋白抗体 |
| 别 名 | YWHAE(phospho T232); 14-3-3 epsilon (phospho Thr232); 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 14-3-3ε; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE. |
| 规格价格 | 100ul/1580元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 免疫学 神经生物学 信号转导 干细胞 细胞凋亡 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 29kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human 14-3-3E around the phosphorylation site of Thr232:LW(p-T)SD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Subunit: Homodimer. Heterodimerizes with YWHAZ. Interacts with NDEL1, RGNEF and TIAM2. Interacts with HCV core protein. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with phosphorylated GRB10. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2. Subcellular Location: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Similarity: Belongs to the 14-3-3 family. SWISS: P62258 Gene ID: 7531 Database links: Entrez Gene: 7531 Human Entrez Gene: 282125 Cow Entrez Gene: 22627 Mouse Entrez Gene: 29753 Rat Omim: 605066 Human SwissProt: P62261 Cow SwissProt: P62258 Human SwissProt: P62259 Mouse SwissProt: P62260 Rat Unigene: 513851 Human Unigene: 234700 Mouse Unigene: 471625 Mouse Unigene: 4225 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Blank control (blue line): MCF7,fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 epsilon (Thr232) antibody (bs-5590R),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. |
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文献和实验antiserum specific for the C terminus of epsilon isoform, protease digestion of intact 14-3-3 showed that the N-terminal half of 14-3-3 (a 16 kDa fragment) was an intact, dimeric domain of the protein. Two isoforms of 14-3-3, tau and epsilon, were expressed
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
Introduction of human 14-3-3 proteins
for the C terminus of epsilon isoform,protease digestion of intact 14-3-3 showed that the N-terminal half of 14-3-3 (a 16 kDa fragment)was an intact,dimeric domain of the protein.Two isoforms of 14-3-3,tau and epsilon,were expressed in E.coli
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