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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Propidium Iodide
- 保质期:
1年
- 供应商:
上海沪震实业有限公司
- 保存条件:
-20℃避光保存,避免反复冻融。孵育缓冲液2-8℃保存
- 规格:
100T
Propidium Iodide染色液
产品概述
-
储存条件:
4℃避光保存。长期保存-20℃避光保存。
Propidium Iodide染色液有效期:
六个月。
Propidium Iodide染色液产品简介:
PI 染色液可用于细胞凋亡检测,也可用于普通的细胞核染色以及某些特定情况下的双链DNA 染色,染色后可用荧光显微镜检测或流式细胞仪检测。Propidium Iodide是一种DNA 结合性染料,其激发和发射波长分别为488nm 和630nm,产生红色荧光,但无膜通透性,不能透过活细胞膜,只能染死细胞。因此,在荧光显微镜下观察,正常细胞不能着色,早期凋亡细胞呈微弱红光,晚期凋亡细胞红光加强,坏死细胞呈强红色荧光。也可采用流式细胞仪利用细胞形态上的改变影响它们的光散射特性改变进行分析,还可与RNase A结合进行细胞周期分析。
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文献和实验High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
of propidium iodide (PI) fluorescence analysis and cell counting performed by the flow cytometer. Only 2 µl is aspirated from a culture for analysis so that assays can be performed in small volumes to minimize reagent cost and usage.
Genome Size Determination Using Flow Cytometry of Propidium Iodide-Stained Nuclei
. Propidium iodide (PI) staining with flow cytometry is a powerful method for genome sizing because it is relatively fast, works with a wide variety of materials, and provides information on a very large number of nuclei. In this method, nuclei are stained
The Use of Propidium Iodide to Assess Excitotoxic Neuronal Death in Primary Mixed Cortical Cultures
in a variety of these pathologies. A key to testing such drugs is the use of a fast, reliable, and easily reproducible model of neurodegeneration and neuroprotection. Our laboratory has previously used propidium iodide (PI) to assess
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