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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见产品说明书
- 库存:
大量
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- CAS号:
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- 英文名:
Leptospira Enrichment, lyophilized, 10 mL vial
- 规格:
10
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文献和实验实验试剂 1. Buffer A 10mM HEPES 1.5mM MgCl2 10mM KCl 0.5mM DTT 0.05% NP40 (or 0.05% Igepal or Tergitol) pH 7.9 2. To prepare 250ml stock of Buffer A:
in the same volume of Buffer 1 as the initial volume transferred from the vial in step 2. 2. Sample Preparation Preparation of MNC from Buffy Coat to Obtain Low Platelet Numbers 1) Dilute 10 – 18 ml buffy coat with Buffer 2 to a total
Monocytes/Macrophages: Enrichment of PBMCs with Monocytes
and transfer it into a 50 ml tube. Fill the tube with PBS, centrifuge 10 min at 300 x g. Resuspend good in 50 ml PBS, centrifuge 10 min at 200 x g. Resuspend good in 50 ml PBS, centrifuge 10 min at 300 x g. Prepare continuous Percoll
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