- ¥1380 - 2200
- LMAI Bio
- LM-5148R
- 进口/国产
- 2026年04月21日
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-5148R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Cdc7
- 抗体名：
细胞分裂周期蛋白7抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 免疫原：
KLH conjugated synthetic peptide derived from human Cdc7:151-250/574
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | Cdc7 |
| 中文名称 | 细胞分裂周期蛋白7抗体 |
| 别 名 | Cdc 7; Cdc-7; Cdc7; CDC7 cell division cycle 7; CDC7 cell division cycle 7 like 1; CDC7 L1; Cdc7 like 1; CDC7 related kinase; CDC7L 1; CDC7L1; Cell division cycle 7 homolog; Cell division cycle 7 kinase; Cell division cycle 7 like protein 1; Cell division cycle 7 related protein kinase; HsCDC 7; HsCDC7; Hsk 1; Hsk1; HuCDC 7; CDC7_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 免疫学 信号转导 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cdc7:151-250/574 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair. Function: Seems to phosphorylate critical substrates that regulatethe G1/S phase transition and/or DNA replication. Canphosphorylates MCM2 and MCM3. Subunit: Forms a complex with either DBF4/DBF4A or DBF4B, leadingto the activation of the kinase activity. Subcellular Location: Nucleus. Similarity: elongs to the protein kinase superfamily. Ser/Thrprotein kinase family. CDC7 subfamily. Contains 1 protein kinase domain. SWISS: O00311 Gene ID: 8317 Database links: Entrez Gene: 8317 Human Omim: 603311 Human SwissProt: O00311 Human Unigene: 533573 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cdc7 Polyclonal Antibody, Unconjugated(bs-5148R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-Cdc7/FITC Conjugated antibody (bs-5148R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. |
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文献和实验的作用。例如，组蛋白 H3N 端的磷酸化可能促进染色质在有丝分裂期间的凝集。在哺乳动物中，aurora B 是有丝分裂时 H3S10 磷酸化的激酶，但是在存在 aurora B 对 H3S10 的磷酸化是不够的。牛痘苗相关激酶 1 是哺乳动物 NHK1 的同系物，它能在体内和体外直接使组蛋白 H3T3 和 H3S10 磷酸化，而失去 VPK1 的活性，组蛋白 H3 的磷酸化也将减少。 组蛋白 H1 被细胞周期蛋白依赖的磷酸化是其翻译后主要的修饰作用。组蛋白 H1 的磷酸化能够影响 DNA 二级
后期促进复合物(anaphase-promoting complex,APC)
APC即遍在蛋白连接酶(ubiquitin ligase, E3)复合物。 E3通常是一种复合体,由多亚基组成。例如从非洲爪蟾卵细胞中分离的周期蛋白B的E3至少含有8个不同的亚基。APC激发E2-遍在蛋白复合物同有丝分裂周期蛋白破坏框结合, 然后激发遍在蛋白同破坏框C-末端的赖氨酸残基结合，此过程不断循环使遍在蛋白多聚化。
Cdc2蛋白是裂殖酵母cdc2基因编码的蛋白，分子两34kDa,所以有称为p34cdc2 蛋白。通过对分离的裂殖酵母突变体的研究发现, 没有Cdc2的活性,细胞不能进入有丝分裂。这表明Cdc2是裂殖酵母进入有丝分裂的一个关键调节因子。实际上它是一种蛋白激酶，与周期蛋白结合后被激活，并促使细胞进入细胞周期。由于裂殖酵母中只有Cdc2一种蛋白激酶，所以p34cdc2 蛋白又被称为细胞周期引擎。
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