- ¥1380 - 2200
- LMAI Bio
- LM-4973R
- 进口/国产
- 2026年01月23日
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Chicken, Cow, Rabbit,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-4973R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Laminin alpha 1
- 抗体名：
层粘蛋白α1抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Chicken, Cow, Rabbit,
- 免疫原：
KLH conjugated synthetic peptide derived from human Laminin alpha 1:2201-2400/3075
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | Laminin alpha 1 |
| 中文名称 | 层粘蛋白α1抗体 |
| 别 名 | LAMA; LAMA1; Laminin A chain; Laminin subunit alpha-1; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-laminin subunit alpha; S-LAM alpha; Laminin subunit alpha-1; LAMA1_HUMAN; Laminin-1 subunit alpha; Laminin-3 subunit alpha. Laminin α-1; Laminin α1; Laminin α 1; Laminin subunit α1; Laminin subunit α-1. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞粘附分子 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Cow, Rabbit, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 337kDa |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Laminin alpha 1:2201-2400/3075 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]. Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Subunit: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-1 is a subunit of laminin-1 (laminin-111 or EHS laminin) and laminin-3 (laminin-121 or S-laminin). Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. Similarity: Contains 17 laminin EGF-like domains. Contains 5 laminin G-like domains. Contains 2 laminin IV type A domains. Contains 1 laminin N-terminal domain. SWISS: P25391 Gene ID: 284217 Database links: Entrez Gene: 10319 Human Entrez Gene: 284217 Human Entrez Gene: 3908 Human Entrez Gene: 3912 Human Entrez Gene: 3913 Human Entrez Gene: 3915 Human Entrez Gene: 3918 Human Entrez Gene: 16772 Mouse Entrez Gene: 316758 Rat Omim: 150320 Human SwissProt: P07942 Human SwissProt: P24043 Human SwissProt: P25391 Human SwissProt: P55268 Human SwissProt: Q9Y6N6 Human SwissProt: P19137 Mouse SwissProt: Q9R0B6 Mouse Unigene: 201805 Human Unigene: 270364 Human Unigene: 302362 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Laminin alpha 1) Polyclonal Antibody, Unconjugated (bs-4973R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Laminin alpha 1) Polyclonal Antibody, Unconjugated (bs-4973R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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文献和实验大鼠海马体神经元和活体大鼠脑注射 α 突触核蛋白后做的免疫细胞化学和免疫组化分析。 原代大鼠海马体神经元被 1 型突触核蛋白前体原纤维（SPR-322）4 µg/mL（D-F）处理后显示路易体内含物的形成，而当被 2 型突触核蛋白前体原纤维（SPR-317）4 µg/mL（A-C）处理时没有路易体内含物形成。组织：原代海马体神经元，物种：斯普拉-道来氏大鼠，固定：由 PFA 制作的 4% 甲醛。一抗：小鼠抗 pSer129 抗体, 稀释度 1:1,000，4 °C 下处理 24 小时；二抗
CD49a/CD49b/CD49c/CD49d/CD49e/CD49f分子
originally identified on activated human T cells, later on fibroblasts, platelets, others. Six forms, VLA1 to VLA6, have been identified, each with distinct alpha chain (numbered alpha-1 to alpha-6) associated with a common beta chain. CD49a - Laminin
（3）同上。两个磷酸化位点都要做，但是可能在不同的病理条件下，侧重点有所不同，这主要体现在你的论文的讨论中。因此建议实验前多做论文研究，查阅一下别人的发表文章，看看与你的病理模型更相关的是哪个位点。 真爱满行囊 非常感谢你 的回复，现在我遇到的问题是我做出来蛋白总量还有216位点是有变化的，9位因为买的是santa curz的抗体，现在死活做不出来，很头痛，我要说明的问题是他的活性降低，我也看到216位点磷酸化水平降低了，可是现在没有第九位的结果（就是没有办法得到第九
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