产品封面图

MPO髓过氧化物酶抗体

收藏
  • ¥780 - 2200
  • LMAI Bio
  • LM-4943R
  • 进口/国产
  • 2025年12月02日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Dog, Horse, Rabbit, Guinea Pig,
avatar
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-4943R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      MPO

    • 抗体名

      髓过氧化物酶抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Dog, Horse, Rabbit, Guinea Pig,

    • 免疫原

      KLH conjugated synthetic peptide derived from human Myeloperoxidase heavy chain:678-745/745

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      50ul  100ul  200ul

    MPO髓过氧化物酶抗体
    英文名称 MPO
    中文名称 髓过氧化物酶抗体
    别    名 Myeloperoxidase; MPO; 89 kDa myeloperoxidase; 84 kDa yeloperoxidase; Myeloperoxidase heavy chain; EC 1.11.1.7; PERM_HUMAN.  
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 肿瘤  心血管  细胞生物  免疫学  激酶和磷酸酶  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Horse, Rabbit, Guinea Pig, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 84kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human Myeloperoxidase heavy chain:678-745/745 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008].

    Function:
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

    Subunit:
    Tetramer of two light chains and two heavy chains.

    Subcellular Location:
    Lysosome.

    DISEASE:
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

    Similarity:
    Belongs to the peroxidase family. XPO subfamily.

    SWISS:
    P05164

    Gene ID:
    4353

    Database links:

    Entrez Gene: 4353 Human

    Entrez Gene: 17523 Mouse

    Entrez Gene: 303413 Rat

    Omim: 606989 Human

    SwissProt: P05164 Human

    SwissProt: P11247 Mouse

    Unigene: 458272 Human

    Unigene: 4668 Mouse

    Unigene: 47782 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片1
    Sample: 
    Spleen (Mouse) Lysate at 40 ug
    Primary: Anti-MPO (bs-4943R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 77 kD
    Observed band size: 125 kD

    产品细节图片2
    Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MPO) Polyclonal Antibody, Unconjugated (bs-4943R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-MPO Polyclonal Antibody, Unconjugated(bs-4943R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    产品细节图片4
    Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-MPO Polyclonal Antibody, Unconjugated(bs-4943R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • 过氧化物酶(MPO)ELISA试剂盒 说明书

      上海西唐生物科技有限公司   021-55229872,  65333639   www.westang.com 人过氧化物酶 ( MPO )ELISA 试剂盒   ( 用于血清、血浆、细胞培养上清液和唾液、尿液、生物体液内 )   原理 本实验采用双抗体夹心  ABC-ELISA 法。用抗人   MPO   单抗包被于酶标板上,标准品和样品中的   MPO与单抗结合,加入生物素化的抗人 MPO ,形成

    • 过氧化物酶(MPO)ELISA试剂盒 说明书

      上海西唐生物科技有限公司   021-55229872,  65333639   www.westang.com 人过氧化物酶 ( MPO )ELISA 试剂盒   ( 用于血清、血浆、细胞培养上清液和唾液、尿液、生物体液内 )   原理 本实验采用双抗体夹心  ABC-ELISA 法。用抗人   MPO   单抗包被于酶标板上,标准品和样品中的   MPO与单抗结合,加入生物素化的抗人 MPO ,形成

    • 过氧化物酶(MPO)酶联免疫分析(ELISA)

      过氧化物酶 (MPO) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用         目的:本试剂盒用于测定人血清,血浆及相关液体样本中 过氧化物酶(MPO) 的 活性。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 人 过氧化物酶 (MPO) 水平。用纯化的 人 过氧化物酶 (MPO) 抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中加入 过氧化物酶 (MPO

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    询价
    上海古朵生物科技有限公司
    2025年07月14日询价
    询价
    上海岑特生物科技有限公司
    2025年07月16日询价
    询价
    上海钰博生物科技有限公司
    2025年06月30日询价
    ¥780
    上海圻明生物科技有限公司
    2025年07月11日询价
    MPO髓过氧化物酶抗体
    ¥780 - 2200