- ￥6022
- Swant
- 瑞士
- PV27-200 µl
- 2025年07月10日
- Rabbit
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
Reconstitute with 200 µl bi-distilled water and make small portions upon arrival (e.g. 2-5 µl). For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep the diluted antiserum at 4°C (with 0.01% Na-azide).
- 克隆性：
多克隆
- 适应物种：
Rabbit
- 供应商：
安诺伦（北京）生物科技有限公司
- 靶点：
Parvalbumin
- 规格：
200 µl
研究领域：Parvalbumin
存储溶液：Lyophilized antiserum (no preservatives).
参考文献：1. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press2. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-3023. Celio M.R., Heizmann C.W. (1982) Nature 297:504-5064. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-4035. *Filice F, Celio M.R., Szabolcsi V. (2017) JCN, in press
应用：Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:500 - 1:1'000 We recommend that the optimal dilutions be determined by titration experiments.
官网链接：https://www.swant.com/pdfs/PV27_Rabbit_anti_Parvalbumin.pdf
商品关键词：Rabbit anti-parvalbumin ,PV27-200 µl / 500 µl / 1 ml,Swant
简单描述：This antiserum was produced against recombinant rat parvalbumin. It cross-reacts with many other species, humans included. It can be used in immunohistochemistry (Fig. 1) and for immunoblotting (Fig. 2).
Lot No: 2014
Reconstitution: with 200 µl / 500 µl / 1 ml bi-distilled water (depending on the volume you ordered).
Immunoblot: The antiserum PV27 recognizes the antigen at 12 kDa after SDS-gel electrophoretic separation of brain extracts. Therefore, antiserum PV27 can be used in immunoblots (Fig. 2).
Immunohistochemistry on Calbindin D-28k knock-out mice: Antiserum PV27 labels a subpopulation of neurons in the normal brain with high efficiency (Fig. 1a), but does not stain the brain of parvalbumin knock-out mice (Fig. 1b).
Product: Antiserum
Background: Calcium binding-proteins represent a family of small, acidic proteins equipped with peculiar cavities which accept Ca2+ with high selectivity (1). There are two types of calcium binding-proteins, “trigger” and “buffer” proteins. Those of the “trigger”-type (e.g. calmodulin and troponin-C) act by changing shape upon binding Ca2+. This distortion exposes regions on the surface of the protein, which interact with surrounding target molecules, altering their activity. The calcium binding-protein of the “buffer”-type are conceived as a system which is in charge of controlling the Ca2+ concentration inside cells. Parvalbumin occurs mainly in subpopulations of nerve cells (2) and in fast muscle fibers (3). It might confer on these cells peculiar skills in the handling of calcium-ions.
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
从鱼类、两栖类到脊椎动物的肌肉中广泛分布，分子量约为 12， 000的酸性蛋白。为一分子有二个 Ca2＋ 的钙结合蛋白质。由 108个氨基酸残基所形成的鲤的细小清蛋白（ parvalbumin），其三维结构通过 X射线衍射法已弄清楚（ Kretsinger， 1973）。从氨基酸排列的相似性来看，认为是肌宁蛋白 C在进化上的原始型，用来研究蛋白与 Ca2＋ 结合后结构变化的模型。
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
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