Reconstitute with 200 µl bi-distilled water and make small portions upon arrival (e.g. 2-5 µl). For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep the diluted antiserum at 4°C (with 0.01% Na-azide).
应用:Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:500 - 1:1'000 We recommend that the optimal dilutions be determined by titration experiments.
简单描述:This antiserum was produced against recombinant rat parvalbumin. It cross-reacts with many other species, humans included. It can be used in immunohistochemistry (Fig. 1) and for immunoblotting (Fig. 2).
Lot No: 2014
Reconstitution: with 200 µl / 500 µl / 1 ml bi-distilled water (depending on the volume you ordered).
Immunoblot: The antiserum PV27 recognizes the antigen at 12 kDa after SDS-gel electrophoretic separation of brain extracts. Therefore, antiserum PV27 can be used in immunoblots (Fig. 2).
Immunohistochemistry on Calbindin D-28k knock-out mice: Antiserum PV27 labels a subpopulation of neurons in the normal brain with high efficiency (Fig. 1a), but does not stain the brain of parvalbumin knock-out mice (Fig. 1b).
Product: Antiserum
Background: Calcium binding-proteins represent a family of small, acidic proteins equipped with peculiar cavities which accept Ca2+ with high selectivity (1). There are two types of calcium binding-proteins, “trigger” and “buffer” proteins. Those of the “trigger”-type (e.g. calmodulin and troponin-C) act by changing shape upon binding Ca2+. This distortion exposes regions on the surface of the protein, which interact with surrounding target molecules, altering their activity. The calcium binding-protein of the “buffer”-type are conceived as a system which is in charge of controlling the Ca2+ concentration inside cells. Parvalbumin occurs mainly in subpopulations of nerve cells (2) and in fast muscle fibers (3). It might confer on these cells peculiar skills in the handling of calcium-ions.