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- 保存条件:
Reconstitute with 10 ul bidistilled water. Freeze in small aliquots (e.g. 1 ug) and keep at -80°C. Avoid repeated freezing and thawing.
- 供应商:
安诺伦(北京)生物科技有限公司
- 规格:
10 µg
靶点/蛋白质:bovine S-100 ß (recombinant) produced in Escherichia Coli.
来源:Recombinant VUSB-1
存储溶液:lyophilized (10 ug in 10 ul of: 10 mM Tris-HCl, 1 mM EGTA).
官网链接:https://www.swant.com/pdfs/S-100-beta.pdf
商品关键词:S-100ß,rS100,Swant
简单描述:This recombinant protein has been purified by HPLC. This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedure:- Dilute 1 µl of the antibody 36 (Swant) against S-100ß in 5 ml of the usual buffer for immunohistochemistry (finaldilution 1:5'000).- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.- Incubate for at least 6 hours in the cold.- Apply to tissue-sections and incubate for 3 days.- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.
Purification: HPLC
Lot No: 63088
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
企业QQ: 3198592576、3212874516
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
2 and EGTA, and make up to 100ml with H2O. The solution can be stored at 40 C 1-2 weeks. Rinse in many changes of PBS. Residual fixative can inhibit the enzyme. Rinsing overnight is fine, but waiting for several days at this step may decrease ß
Crystallization of Kinesin Family Motor Proteins
) PEG 4000, 2.5 mM ATP, 10 mM MgCl2Reservoir: 100 mM Na-acetate pH 4.6, 150 mM KCl, 7% PEG 4000, 5 mM ATP, 20 mM MgCl2Kull et al., 1996Ncd 335-700Sitting drop Room temperature7 mg/ml protein in 10 mM Pipes, pH 7.2, 100 mM NaCl, 1 mM EGTA, 1 mM DTT, 7% (w
. A sample was run on a 15% non-denaturing acrylamide gel for visualization (2A). HeLa cells were transfected with 100 nM of the 12-15 bp RNase III generated GAPDH siRNA s or a 21 bp chemically synthesized GAPDH siRNA . GAPDH protein levels were monitored
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