Reconstitute with 10 ul bidistilled water. Freeze in small aliquots (e.g. 1 ug) and keep at -80°C. Avoid repeated freezing and thawing.
供应商:
安诺伦(北京)生物科技有限公司
规格:
10 µg
研究领域:Others
靶点/蛋白质:bovine S-100 ß (recombinant) produced in Escherichia Coli.
来源:Recombinant VUSB-1
存储溶液:lyophilized (10 ug in 10 ul of: 10 mM Tris-HCl, 1 mM EGTA).
官网链接:https://www.swant.com/pdfs/S-100-beta.pdf
商品关键词:S-100ß,rS100,Swant
简单描述:This recombinant protein has been purified by HPLC. This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedure:- Dilute 1 µl of the antibody 36 (Swant) against S-100ß in 5 ml of the usual buffer for immunohistochemistry (finaldilution 1:5'000).- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.- Incubate for at least 6 hours in the cold.- Apply to tissue-sections and incubate for 3 days.- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.