After reconstitution, freeze in small aliquots (e.g. 1 to 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.
供应商:
安诺伦(北京)生物科技有限公司
规格:
100 µg
研究领域:Calbindin
靶点/蛋白质:HPLC-purified calbindin D-28k (recombinant, 100 g) produced in Escherichia Coli, after in vitro cDNA synthesis.
来源:Recombinant mouse
存储溶液:100 ug in 250 ul of 20 mM NaHCO3 with high CaCl2 (0.1 mM). Lyophilized.
应用:This protein can be used for immuoblots and absorption experiments. For intracellular injections in neurons we suggest using our other products: AgCB10ic or AgCB100ic.
官网链接:https://www.swant.com/pdfs/AgCB100abs.pdf
商品关键词:Calbindin D-28k,AgCB100abs,Swant
简单描述:This recombinant protein has been purified by HPLC and migrates as a single band of approximately 28 kDa. The figure shows a gel stained with Coomassie-blue.For adsorption experiments aimed at proofing the specificity of the immunostaining, we suggest the following procedure:- Dilute 1 µl of one of the Swants antibodies against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.- Incubate for at least 6 hours in the cold.- Apply to tissue-sections and incubate for 3 days.- Complete the immunohistochemical reaction as usual (biotinylated secondary antibody, Streptavidin-Cy2 (or other fluorescent dyes), or peroxidase (followed by DAB-H2O2).As a result, the immunostaining should be strongly reduced or even completely prevented.
Purification: HPLC
Lot No: 13 (20-17)
Reconstitution: with 250 ul of bi-distilled water.