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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-3696R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
AF10
- 抗体名:
髓系、淋巴、混合系白血病易位基因10抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human AF10/MLLT10:151-250/1027
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /Test (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | AF10 |
| 中文名称 | 髓系、淋巴、混合系白血病易位基因10抗体 |
| 别 名 | AF 10; AF10; AF10_HUMAN; ALL 1 fused gene from chromosome 10; ALL1 fused gene from chromosome 10; ALL1-fused gene from chromosome 10 protein; MLLT 10; MLLT10; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10; Myeloid/lymphoid or mixed lineage leukemia translocated to 10; Protein AF 10; Protein AF-10; Protein AF10; Type I AF10 protein. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /Test (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 109kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AF10/MLLT10:151-250/1027 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. Function: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. Subunit: Self-associates. Interacts with FSTL3 isoform 2; the interaction enhances MLLT10 in vitro transcriptional activity and self-association. Interacts with YEATS4. Interacts with SS18. Interacts with DOT1L. Subcellular Location: Nucleus. Tissue Specificity: Expressed abundantly in testis. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Note=A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. Note=A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. Similarity: Contains 2 PHD-type zinc fingers. SWISS: P55197 Gene ID: 8028 Database links: Entrez Gene: 8028 Human Entrez Gene: 17354 Mouse Omim: 602409 Human SwissProt: P55197 Human SwissProt: O54826 Mouse Unigene: 30385 Human Unigene: 209175 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Testis (Rat) Lysate at 40 ug Primary: Anti-AF10 (bs-3696R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 109 kD Observed band size: 109 kD ![]() Blank control: U-2OS(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). |
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文献和实验急性混合细胞白血病依白血病细胞来源及表达不同分两种类型: 1.双表型:在混合细胞白血病中,确定有大于或等于10%的恶变细胞,既有淋巴细胞系,又有髓细胞系特性。 2.双系型:也可命名为双克隆型(同时有两种或多种分别表达髓系或不同淋巴系标记的白血病细胞)。检验包括细胞形态学(Auer小体)、细胞化学(包括免疫细胞化学及超微结构)、单克隆抗体医学教育|网搜集整理、E玫瑰花结、SmIg、C1q、遗传及分子生物学。依据以上多方面检测,进行综合判断,才能诊断本病。大多数病人有t
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