手机验证
Human Serum
ELISA
上海研卉生物科技有限公司
现货
480T
Cat. No. | Components | Size (480 tests) |
PK401-213 | Human HER-2 | 10 μg |
PK402-213 | Biotinylated anti-HER-2 antibody | 10 μg |
PK403-213 | Streptavidin-HRP | 10 μg |
PK404-213 | Anti-HER-2 antibody | 30 μg |
How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development.
HER-2 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Herceptin®. Many investigational anti-HER-2 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.
The enclosed ELISA assay kit for anti-HER-2 h-mAb in human serum is based on an enzyme immunoassay (ELISA) between recombinant HER-2 protein (ECD) and a biotinylated anti-HER-2 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-HER-2 antibodies in human serum.
The assay only involves the following steps:
1. Coat the plate with human HER-2;
2. Add the mixture of your sample and biotinylated anti-HER-2 antibody;
3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;
4. Record the OD readings and analyze the serum concentration.
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Wide Coverage
There is no requirement for the species and subtype of the antibody.
Easy Process
There is no longer a need to generate anti-drug antibodies for PK studies.
Clean Result
The use of the biotinylated antibody in the competitive ELISA significantly alleviates the issue of background noise issue that often occurs in traditional ELISA method.
Upon receipt, please store all items at -20℃.
After reconstitution, the stock solution should be kept at -80℃.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Inhibition of HER-2: biotinylated anti-HER-2 antibody binding by an anti-HER-2 antibody.
Serial dilutions of the Therapeutic Anti-HER-2 Antibody (1:2 serial dilutions, from 20 μg/mL to 0.039 μg/mL) was added into HER-2: Biotinylated Anti-HER-2 Antibody binding reactions.
药物代谢动力学 (Pharmacokinetic, PK) 是定量研究药物在生物体内吸收、分布、代谢和排泄规律,并运用数学原理和方法阐述血药浓度随时间变化规律的一门学科。血药浓度-时间曲线,简称为药-时曲线,是指血药浓度随时间变化的动态过程,反应药物在体内的代谢情况,药时曲线下的面积 (Area under curve, AUC) 代表一次用药后的吸收总量,反映了药物的吸收程度。由于血液是药物及其代谢物在体内吸收、分布、代谢和排泄的媒介,各种体液和组织中的药物浓度与血液中的药物浓度保持一定的比例关系,所以血药浓度变化最具有代表性,是最常用的样本。为助力抗体药物临床前和临床药代动力学研究,设计一种精准的实验方法测定血药浓度就显得尤为重要。
为了支持上述研究,ACROBiosystems公司开发了一系列ELISA血药浓度定量检测试剂盒,用于测定临床前和临床血液样本中的抗体药浓度。该系列产品经证实具有低背景、通用快速、高批间一致性等卓越的实验表现。
货号 | 产品描述 | 规格 |
---|---|---|
EPH-V1 | ELISA Assay Kit for Anti-PD-1 h-mAb in Human Serum | 96/480 tests |
EPM-V1 | ELISA Assay Kit for Anti-PD-1 h-mAb in Mouse Serum | 96/480 tests |
EPC-V1 | ELISA Assay Kit for Anti-PD-1 h-mAb in Monkey Serum | 96/480 tests |
EHH-V1 | ELISA Assay Kit for Anti-HER-2 h-mAb in Human Serum | 480 tests |
EHM-V1 | ELISA Assay Kit for Anti-HER-2 h-mAb in Mouse Serum | 480 tests |
EHC-V1 | ELISA Assay Kit for Anti-HER-2 h-mAb in Monkey Serum | 480 tests |
ECH-V1 | ELISA Assay Kit for Anti-CTLA-4 h-mAb in Human Serum | 96/480 tests |
ECM-V1 | ELISA Assay Kit for Anti-CTLA-4 h-mAb in Mouse Serum | 96/480 tests |
ECC-V1 | ELISA Assay Kit for Anti-CTLA-4 h-mAb in Monkey Serum | 96/480 tests |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。