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- 2025年11月05日
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- 英文名：
Insulin-Transferrin-Sodium Selenite Supplement
- 供应商：
嵘崴达
- 保存条件：
2-8°C
- 规格：
50 MG
Insulin-Transferrin-Sodium Selenite Supplement
suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)
别名:
ITS
一般描述
The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.
The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
应用
The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.
制备说明
Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
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文献和实验N2 supplement的配置和N2 Supplement操作手册
)后-20保存，应用时拿出一支解冻后按所需浓度(如为100X的，则稀释100倍，即100毫升培养液里加1毫升N2)加到培养液里就行。没有说明书吗?成分说明书上肯定有。我这里给你列出： COMPONENTS: Human Transferrin (Holo) :转铁蛋白 Insulin (Bovine)：胰岛素 Progesterone：黄体酮 Putrescine：腐胺 Selenite：亚硒酸钠 浓度均为1mM
insulin, 5 μg/ml transferrin, 5 ng/ml sodium selenite, 20 ng/ml dexamethasone, 20 ng/ml l-thyroxine, 50 ng/ml bFGF, 100 ng/ml PDGF, and 20 ng/ml EGF. 4. Cells were grown either in plastic dishes or in dishes coated with 5 μg/cm2 fibronectin.
Arachidonic Acid Labeling 花生死烯酸标记方法
serum free media and supplement with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin).--> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml.4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???).--> Make
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