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文献和实验Summary WCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)).TFIIIC complex is first purified by flag affinity: TFC4 contains a single copy of the flag epitope.The flag purified TFIIIC is then further purified by anion exchange
Purification of TFIIIC from yeast
Summary WCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)).TFIIIC complex is first purified by flag affinity: TFC4 contains a single copy of the flag epitope.The flag purified TFIIIC is then further purified by anion exchange
哺乳动物RNAi技术 Mammalian RNA Interference
libraries or mRNA sequences of the respective organism to ensure that only a single gene is targeted. (Although optional, this step is recommended) Synthesize several siRNA duplexes to control for the specificity of the knockdown experiments
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