Human heterogeneous nuclear ri

Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes

class of nuclear pre-mRNA/mRNA-binding proteins, the heterogeneous nuclear ribonucleoproteins (hnRNPs), to form hnRNP complexes (1 ,2 ). The molecular architecture of these complexes must be exceedingly dynamic because each step in the pre-mRNA

An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to Human Telomerase

Antisense oligonucleotides have been used to study the structure and function of small nuclear ribonucleoprotein (snRNP) complexes and were adapted and modified for the purification of a variety of RNPs. We describe methods for recombinant

Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques

Human telomerase is a ribonucleoprotein (RNP) that synthesizes DNA repeats at the ends of chromosomes and maintains telomere length and genome stability. The enzyme comprises telomerase RNA (hTR) (which provides the template for telomere