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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-2409R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Amphiphysin
- 抗体名:
神经元突触前膜蛋白抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit,
- 免疫原:
KLH conjugated synthetic peptide derived from human Amphiphysin:101-200/695
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | Amphiphysin |
| 中文名称 | 神经元突触前膜蛋白抗体 |
| 别 名 | Amphiphysin; Amp; AMPH 1; AMPH1; Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen); Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); Amphiphysin I; CG8604; Damp; dAmph; Dmel\CG8604; HGNC:471; MGC49429; Stiff man syndrome with breast cancer 128kD autoantigen; Stiff man syndrome with breast cancer 128kDa autoantigen; AMPH_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 76kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Amphiphysin:101-200/695 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. Function: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. Subunit: Heterodimer with BIN1. Binds SH3GLB1 and AP2A2. Interacts with AP2B1. Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Tissue Specificity: Neurons, certain endocrine cell types and spermatocytes. Similarity: Contains 1 BAR domain. Contains 1 SH3 domain. SWISS: P49418 Gene ID: 273 Database links: Entrez Gene: 273 Human Entrez Gene: 218038 Mouse Entrez Gene: 60668 Rat Omim: 600418 Human SwissProt: P49418 Human SwissProt: Q7TQF7 Mouse SwissProt: O08838 Rat Unigene: 592182 Human Unigene: 101650 Mouse Unigene: 44463 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Amphiphysin Polyclonal Antibody, Unconjugated(bs-2409R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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文献和实验2以及Amphiphysin抗体,它们的总阳性率达到60%;抗Hu抗体在亚急性小脑变性以及亚急性感觉神经元病中的阳性率分别为50%和82%。 诊断标准 典型PNS: ① 患有典型综合征(即脑脊髓炎,边缘性脑炎,亚急性小脑变性,斜视性眼阵挛肌阵挛,亚急性感觉神经元病,慢性胃肠道假性梗阻,肌无力综合征或皮肌炎),并在神经系统疾病诊断后的5年内癌症有发展,无论副瘤性抗体是否存在。 ② 患有非典型综合征,在癌症治疗后,症状得到客观的改善或解决,但前提是它本身不易自行缓解。 ③
在突触中,介于突触间隙相对面的突触前纤维末端膜和突触后神经元细胞膜,分别称为突触前膜( P- resynaptic membrane)和突触下膜。在突触后神经元中,把突触下膜以外的部分称为突触后膜( pos- tsynaptic membrane),但有时也把突触下膜称为突触后膜。在电子显微镜下多可看到突触下膜及突触前膜和突触间隙均染色很深。从突触前膜所释放的化学传递物质与突触下膜相结合,并作用于它,使其离子通透性改变,结果发生离子移动,而使突触后膜以电的传导使兴奋性发生
在下一个神经元的胞体或树突表面。在电子显微镜下观察到,突触的接触处有两层膜,轴突末梢的轴突膜称为突触前膜,与突触前膜相对的胞体膜或树突膜则称为突触后膜,两膜之间为突触间隙。一个突触即由突触前膜、突触间隙和突触后膜三部分组成。突触前膜和后膜较一般的神经元膜稍增厚,约7.5nm左右。突触间隙约20nm左右,其间有粘多糖和糖蛋白(图10-2)。在突触前膜内侧有致密突起,致密突起和网格形成囊泡栏栅,其间隙处正好容纳一个囊泡;因此设想,这种栏栅结构具有引导囊泡与突触前膜接触的作用,促进囊泡内递质的释放。在突触
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