Mouse/Human/Rat Phospho-AKT1 (
Ser473) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1447- $740
- LSBio
- 详见官方网站
- LS-F1447
- 美国
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
Mouse/Human/Rat Phospho-AKT1 (Ser473) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1447
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About AKT1
P31749 NM_005163 NP_005154.2AKT1 ElisaKit, AKT ElisaKit, AKT-1 ElisaKit, C-AKT ElisaKit, Pan-AKT ElisaKit, PKB alpha ElisaKit, PKBalpha ElisaKit, Proto-oncogene c-Akt ElisaKit, Protein kinase B ElisaKit, Rac protein kinase alpha ElisaKit, PRKBA ElisaKit, Protein kinase B alpha ElisaKit, RAC-PK-alpha ElisaKit, PKB ElisaKit, PKB-ALPHA ElisaKit, RAC ElisaKit, RAC-ALPHA ElisaKit
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.(More About AKT1)
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
=result&resnum=1#v=onepage&q=gsk%20tyrosine%20phosphorylation%20and%20activity&f=false By the way, based on my own experience, most of antibodies from Santa Cruz are NOT reliable at all. I strongly recommend Cell Signaling Technology (CST
过程中，一抗应在封闭缓冲液 (TBS/0.3%Triton. X–100/5% NGS) 中稀释。抗体：Phospho–Akt (Ser473) (D9E) XP. Rabbit mAb #4060、Phospho–EGFReceptor (Tyr1173) (53A5) Rabbit mAb #4407样本：石蜡包埋人类乳腺肿瘤（上图）和 HCC827 异种移植物（下图）抗体稀释：SignalStain. 抗体稀释液（左）或 TBST/5%NGS（右）检测—检测系统传统的 IHC 检测
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