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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-20689R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Integrin alpha X /CD11c
- 抗体名:
整合素αX抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat,
- 免疫原:
KLH conjugated synthetic peptide derived from human Integrin alpha X /CD11c:301-400/1163 <Extracellular>
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | Integrin alpha X /CD11c |
| 中文名称 | 整合素αX抗体 |
| 别 名 | Integrin alpha-X; integrin alpha-X precursor; ITGAX; CD11C; SLEB6; CD11C (p150) alpha polypeptide; complement receptor 4; integrin aX; CD11 antigen-like family member C; leukocyte adhesion receptor p150,95; leukocyte adhesion glycoprotein p150,95 alpha chain; ITAX_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 126kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Integrin alpha X /CD11c:301-400/1163 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. [provided by RefSeq]. Function: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Subunit: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain. Subcellular Location: Membrane. Tissue Specificity: Predominantly expressed in monocytes and granulocytes. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain. SWISS: P20702 Gene ID: 3687 Database links: Entrez Gene: 3687 Human Entrez Gene: 16411 Mouse Entrez Gene: 499271 Rat Omim: 151510 Human SwissProt: P20702 Human SwissProt: Q9QXH4 Mouse Unigene: 248472 Human Unigene: 22378 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Blank control: Mouse Spleen(fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody:Rabbit Anti-Integrin alpha X /CD11c antibody (bs-20689R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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文献和实验CD11a/CD11b/CD11c 分子 CDlla 常用单克隆抗体或代号:MHM24, 2F12; CRIS―3(LFA―lα链,整合素α1) 主要表达细胞:Leu[A5] 分子质量(kDa)和结构:spl80(整合素α) 功 能:与1CAM-l(CD54)、ICAM-2(CDl02)、ICAM-3(C1350)结合,介导细胞黏附;与JAMl结合,参与白细胞穿过内皮细胞
LABELING ALPHA-ACTININ WITH IATR
with good stirring.The volume (ml)of borate buffer = mg IATR x 9 / (mg/ml)alpha-actinin. 4.Clarify IATR solution at 25,000 rpm,4℃,for 10 min in a 42.2Ti rotor. 5.Mix equal volumes of clarified dye and alpha-actinin in a test tube; remove
ALPHA FACTOR ARREST AND RELEASE
of approximately 3 x 10�5 M, while bar1 strains require much lower pheromone concentrations (10-8 M). To release cells from alpha factor arrest, spin down cells and wash twice (using a large volume, dependent on the size of the cell pellet anticipated
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