Ca2+ promoted Ras inactivator; Calcium promoted Ras inactivator; CAPRI; GAPL; RASL2_HUMAN; GTPase activating protein like; KIAA0538; MGC131890; Ras GTPase activating protein 4; RAS p21 protein activator 4; RasGAP activating like protein 2.
规格价格
100ul/1380元 购买 200ul/2200元 购买 大包装/询价
说 明 书
100ul 200ul
研究领域
细胞生物 信号转导 G蛋白偶联受体 G蛋白信号
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse,
产品应用
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
90kDa
细胞定位
细胞浆
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human RASA4:501-600/803
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function: Ca2+-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis.
Subcellular Location: Cytoplasm; cytosol. Cell membrane; peripheral membrane protein. Note=Localized to the cytosol as a result of its lack of phosphoinositide binding activity. Upon agonist-stimulated calcium mobilization, utilizes the C2A and C2B domains to associate with the plasma membrane.
Tissue Specificity: Widely expressed.
Similarity: Contains 1 Ras-GAP domain.
SWISS: O43374
Gene ID: 10156
Database links:
Entrez Gene: 10156 Human
Entrez Gene: 54153 Mouse
Entrez Gene: 288589 Rat
Omim: 607943 Human
SwissProt: O43374 Human
SwissProt: Q6PFQ7 Mouse
Unigene: 696339 Human
Unigene: 290655 Mouse
Unigene: 1366 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample:Spleen (Mouse)Lysate at 40 ug Primary: Anti-RASA4(bs-19732R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 90kD Observed band size: 90kD
Sample: HepG2 Cell (Human) Lysate at 40 ug MCF-7 Cell (Human) Lysate at 40 ug Lovo Cell (Human) Lysate at 40 ug Primary: Anti-RASA4(bs-19732R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90kD Observed band size: 90kD