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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
RNase H
- 保质期：
一年
- 供应商：
上海信裕生物科技有限公司
- 保存条件：
-20℃恒温保存，有效期一年
- 规格：
600U
【产品概述】
RNase H是一种核酸内切酶，可特异性地水解与DNA杂交的RNA的磷酸二酯键，产生具有游离5’-P和3’-OH末端的产物。该酶不能降解单链核酸、双链DNA或双链RNA。
【用途】
| RNase H (60 U/μl) | 10 μl |
| 5x RNase H Buffer | 300 μl |
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文献和实验RNase H: Specificity, Mechanisms of Action, and Antiviral Target
The Ribonuclease (RNase) H is one of the four enzymes encoded by all retroviruses, including HIV. Its main activity is the hydrolysis of the RNA moiety in RNA–DNA hybrids. The RNase H ribonuclease is essential in the retroviral life cycle
Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
Question 1.What are the differences among RNase H, RNase A, RNase B and RNase C? 2.In your cDNA kits, RNase H is added in the second strand reaction to produce more nicked RNA as primers for DNA synthesis. In this repect
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