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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
StarScript II Green One-Step qRT-PCR Kit UNG
- 保质期:
一年
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
-20℃恒温保存,有效期一年
- 规格:
100 rxn/500 rxn/1000 rxn
【产品概述】
StarScriptII Green One-step qRT-PCR Kit 是采用SYBR Green I 嵌合荧光法进行一步法实时荧光定量检测的专用试剂盒。使用本制品进行Real Time RT-PCR反应时以提取的RNA为模版,在同一反应管内连续进行反转录和荧光定量检测,操作简单,并能有效防止污染。本产品基于高效的StarScript II反转录酶、抗体型HotstartTaqDNA聚合酶配合优化的Buffer体系研制,在提高检测灵敏度的同时保证了扩增的特异性;非常适合于RNA病毒等微量目标基因的检测。
本产品使用时只需加入模板、引物、ROX Reference Dye(用以校正孔与孔之间产生的荧光信号误差,根据不同荧光定量PCR仪选择使用)和水,使其工作浓度为1×,即可进行反应。具有快速简便、灵敏度高、特异性强、稳定性好等优点,可最大限度地减少人为误差、节约PCR实验操作时间、降低污染几率。
【产品组分】
| 产品组分 | XY326-01 | XY326-05 | XY326-10 |
| StarScript II One-Step Enzyme Mix(UNG) | 200 μl | 1 ml | 1 ml×2 |
| 2×StarScript II One-Step qRT-PCR Buffer(dUTP) | 1 ml | 1 ml×5 | 5 ml×2 |
| DEPC-ddH2O | 1 ml | 1.5 ml×3 | 5 ml×2 |
| ROX Reference Dye | 40 μl | 200 μl | 400 μl |
| ROX Reference Dye II | 40 μl | 200 μl | 400 μl |
*注:不同仪器所需ROX Reference Dye不同:
需加ROXReference Dye (50×)的机型:ABI Prism7000/7300/7700/7900HT和ABI Step One /ABI Step One Plus。
需加ROXReference Dye II (50×)的机型:ABI Prism7500 /7500 Fast , MJ Research Chromo4,Opticon (II) , Corbett Rotor Gene 3000。
无需加ROX Reference Dye的机型: Thermal Cycler Dice Real Time System , LightCycler , Smart Cycler System,Corbett Rotor-gene 6000, Agilent Technologies Mx3000P等荧光定量PCR仪。
【保存条件】
-20℃恒温保存,有效期一年
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