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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
StarScript II SYBR Two-Step qRT-PCR Synthesis Kit(with ROX)
- 保质期:
一年
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
-20℃恒温保存,有效期一年
- 规格:
20 rxn/50 rxn
【产品概述】
|
组分名称
|
XY308-02
|
XY308-05
|
|
StarScript II RT Mix
|
20 μl
|
50 μl
|
|
2×RT Reaction Mix(With Primer)
|
200 μl
|
500 μl
|
|
gDNA Remover
|
10 μl
|
25 μl
|
|
5×gDNA Remover Reaction Mix
|
100 μl
|
250 μl
|
|
2×RealStar Green Fast Mixture with ROX
|
1.1 ml
|
1.1× 3ml
|
|
DEPC-ddH2O
|
1 ml
|
2.5 ml
|
注:不同仪器所需ROX Reference Dye不同,如需添加,需致电单独配带:
ROX Reference Dye (50×):ABI Prism7000/7300/7700/7900HT和ABI Step One /ABI Step One Plus荧光定量PCR仪。
ROX Reference Dye II (50×):ABI Prism7500/7500 Fast荧光定量PCR仪 , MJ Research Chromo4,Opticon (II) , Corbett Rotor Gene 3000荧光定量PCR仪。
不加ROX Reference Dye: Thermal Cycler Dice Real Time System , LightCycler , Smart Cycler System,Corbett Rotor-gene 6000, Agilent Technologies Mx3000P荧光定量PCR仪等。
【保存条件】
-20℃恒温保存,有效期一年
1. 使用前请将各组分上下颠倒轻轻混匀,尽量避免起泡,并经短暂离心后使用。
2. 尽可能减少2×RealStar Green Fast Mixture在光下的曝露时间,长时间的曝光可导致荧光信号强度的丧失。
3. 本试剂盒不能用于杂交探针法。
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文献和实验. Drawback: rapidly dividing cells will have more rRNA and different rRNA/mRNA ratio which will complicate comparison; difference in cDNA synthesis not taken into account. Genomic DNA Genomic DNA or cell number. Drawbacks: RNA degrades faster than RNA
and synthesis of first-strand cDNA (ref. 60), allowing the construction of solid-phase cDNA libraries and solid-phase RT-PCR. 实验试剂 Lysis/Binding Buffer Washing Buffer A Washing Buffer B 10 mM Tris-HCl (Elution Buffer
Real-Time qRT-PCR standard curves... efficiency is too h
Greetings, In my Real-Time qRT-PCR experiments, I employ the standard curve method for quantification of gene expression. However, standard curves seem to be a huge hit-or-miss procedure for me, even with genes that are well-established
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