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北京博蕾德生物科技有限公司
- 规格:
4024T/96 tests/96T
| 规格: | 4024T | 产品价格: | ¥3928.0 |
|---|---|---|---|
| 规格: | 96 tests | 产品价格: | 询价 |
| 规格: | 96T | 产品价格: | 询价 |
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文献和实验Strategies to Optimize Protein Expression in E. coli
. Biol. Chem. 277:50564‐50572. Phillips, T.A., Vanbogelen, R.A., and Neidhardt, F.C. 1984. Ion gene‐product of Escherichia coli is a heat‐shock protein. J. Bacteriol. 159:283
v468 Chapter 9 自身启动子报告基因验证转录靶点
.Then add 5 μL of the ligation mix and resuspend gently. 3. Incubate on ice for 30 min. 4. Heat shock for 20 sec at 37°C. 5. Incubate on ice for 2 min. 6. Add 500 μL SOC medium and incubate on a shaker at 300rpm for 1 h at 37°C.
Stripping and reprobing on Immobilon-P membrane using low pH and heat/detergent methods. (A) Membrane blots of rat liver lysate were probed with rabbit anti-ERK-1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20
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