Mouse Receptor Activator Of Nu

RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells

or indirectly. This protocol describes a procedure for generating osteoclasts from mouse bone marrow cells using macrophage colony stimulating factor and receptor activator of NF-κB ligand.

Rho GTPase Techniques in Osteoclastogenesis

. Since the identification and cloning of receptor activator of nuclear factor kappa B ligand (RANKL), culturing primary osteoclasts in vitro has become much simplified. It has become apparent that the actin cytoskeleton is extremely important for the osteoclast

Generation of Osteoclasts In Vitro, and Assay of Osteoclast Activity

: receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, we can induce osteoclasts from monocytemacrophage lineage cells even in the absence of osteoblasts. This chapter describes