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- 详细信息
- 文献和实验
- 技术资料
- 库存:
3012
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
3107T/96 tests/3106T/96T
| 规格: | 3107T | 产品价格: | ¥3011.0 |
|---|---|---|---|
| 规格: | 96 tests | 产品价格: | 询价 |
| 规格: | 3106T | 产品价格: | ¥3010.0 |
| 规格: | 96T | 产品价格: | ¥5835.38 |
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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