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- 详细信息
- 文献和实验
- 技术资料
- 库存:
2776
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
2871T/96T
| 规格: | 2871T | 产品价格: | ¥2775.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥4050.0 |
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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