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- 详细信息
- 文献和实验
- 技术资料
- 库存：
2494
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
2589T/96 tests/96T
| 规格： | 2589T | 产品价格： | ￥2493.0 |
|---|---|---|---|
| 规格： | 96 tests | 产品价格： | 询价 |
| 规格： | 96T | 产品价格： | 询价 |
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
5 above. Fixation for > 5 minutes can lead to decreased enzyme activity. STAINING FOR ALKALINE PHOSPHATASE ACTIVITY Phosphatase genes are useful reporter genes as several histochemical methods that yield precipitated, highly colored and/or electron
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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