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文献和实验PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
;2-adrenergic receptor, insulin-like growth factor receptor-2 (IGFR-2), γ-glutamyl transferase (γ-GT), AQP-4, and VAMP-2, a membrane protein associated with caveolae. Whether or not these molecules are expressed in ATI cells exclusively
Production of Monoclonal Antibody by DNA Immunization with Electroporation
associated with problematic antigens: low abundance and protein instability and use of recombinant proteins that lack posttranslational modifications. This chapter describes the use of a DNA-based immunization protocol for the production of mAb
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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