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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
PCR products, and primers to anested pair of primers specific to the long-terminal repeats ofBXH-2 MuLV and complementary to PrimerLongTA, respectively. Individual secondary PCR bands are resolved on agarose geland subsequently recovered for sequencing
【申请上传】Current Protocols in Immunology
as Adjuvants Unit 2.12 Cloning, Expression, and Modification of Antibody V Regions Unit 2.13 Production of Bispecific Antibodies Unit 2.14 Nucleic Acid Immunizations Chapter 3 In Vitro Assays for Mouse Lymphocyte Function Introduction Section I Isolation
Materials ds cDNA (700 to 1300 ng; see protocol 2 ) QIAquick PCR Purification Kit (Qiagen
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