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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
Wiley & Sons, Inc. Keywords: pronucleus injection; gene targeting; mouse mutant; zinc?finger nuclease; TAL nuclease; homologous recombination
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
harboring the plasmid that encodes the QSSR1 zinc finger fused to fingers 1 and 2 of the Zif268 protein and the Gal4 activation domain formed blue colonies only when mated with cells that contained the GTA and GCA reporter plasmids. This means
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