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北京博蕾德生物科技有限公司
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1217T/96T
| 规格: | 1217T | 产品价格: | ¥1121.0 |
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文献和实验, such as RNA interference, ribozymes, or antisense RNA, that provide solely knock-down phenotypes2, 3 , transcription factor�based tools can generate both loss-of-function phenotypes (when the transcription factor is linked to a repressor domain) and gain
Linear amplification of limiting amounts of RNA for gene expression studies
, our group investigated the role of the paired domain transcription factor Pax2 in brain development (Bouchard et al ., 2005). Pax2 is required for the formation of the isthmic organizer (IsO) at the midbrain-hindbrain boundary, where it initiates expression
that encodes a hybrid transcription factor (Fig. 1A). The hybrid transcription factor consists of two domains: a DNA-binding domain and a transcriptional activation domain. We used the Gal4 transcriptional activation domain fused to the Zif268 protein
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