相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-17769R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
MRPL13
- 抗体名:
线粒体核糖体蛋白L13抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human MRPL13:21-120/178
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | MRPL13 |
| 中文名称 | 线粒体核糖体蛋白L13抗体 |
| 别 名 | 39S ribosomal protein L13, mitochondrial; L13; L13A; L13mt; Mitochondrial ribosomal protein L13; MRP L13; OTTHUMP00000228247; OTTHUMP00000228248; RPL13; RPML13. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 21kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MRPL13:21-120/178 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008] Function: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [Entrez Gene] Subunit: Interacts with OXA1L. Subcellular Location: Mitochondrion Similarity: Belongs to the ribosomal protein L13P family. SWISS: Q9BYD1 Gene ID: 28998 Database links: Entrez Gene: 617901 Cow Entrez Gene: 482029 Dog Entrez Gene: 28998 Human Entrez Gene: 68537 Mouse Entrez Gene: 299938 Rat Entrez Gene: 553555 Zebrafish Omim: 610200 Human SwissProt: Q3SYS1 Cow SwissProt: Q9BYD1 Human SwissProt: Q9D1P0 Mouse Unigene: 333823 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRPL13) Polyclonal Antibody, Unconjugated (bs-17769R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验μg/ml)的TE缓冲液(pH8.0)溶解沉淀,将溶液转移到另-1.5mlEppendorf管中,室温放置1.5mlEppendorf管中30min。 18.加入400μl13%(v/v)的PEG8000-NaCl(1.6mol/L),混匀,4℃12000rpm离心5min以回收质粒DNA,弃去上清。 19.加入400μlTE缓冲液(pH8.O)溶解沉淀,再分别用等体积的Tris饱和酚、酚:氯仿:异戊醇(25:24:1)、和氯仿各抽提一次。 20.将水相(上清
技术资料暂无技术资料 索取技术资料











