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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海康朗生物科技有限公司
- 库存:
大量
- 目录编号:
kl-9514R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
12个月
- 抗体英文名:
PI14 antibody
- 抗体名:
蛋白酶抑制剂14抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Horse,
- 免疫原:
KLH conjugated synthetic peptide derived from human SERPINI2/PI14:151-250/405
- 亚型:
IgG
- 形态:
冻干粉或液体
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul 200ul
| 中文名称 | 蛋白酶抑制剂14抗体 |
| 别 名 | MEPI; Myoepithelium derived serine protease inhibitor; Pancpin; Pancreas specific protein TSA2004; Peptidase inhibitor 14; PI 14; PI14; Protease inhibitor 14; Serine (or cysteine) proteinase inhibitor clade I (neuroserpin) member 2; Serine (or cysteine) proteinase inhibitor clade I (pancpin) member 2; Serpin I2; Serpin peptidase inhibitor clade I (pancpin) member 2; TSA2004; SPI2_HUMAN; SERPINI2. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Horse, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SERPINI2/PI14:151-250/405 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: SERPINI2 is a member of the serine protease inhibitor (serpin) superfamily made up of proteins which play central roles in the regulation of a wide variety of physiological processes, including coagulation, fibrinolysis, development, malignancy and inflammation. It may have a role in a growth-control, possibly growth-suppressing pathway and, when impaired, may be involved in pancreatic carcinogenesis. The protein is a member of the plasminogen activator inhibitor-1 family, a subset of the serpin superfamily whose members act as tissue-specific tPA inhibitors. Two alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. Subcellular Location: Secreted Tissue Specificity: Expressed in pancreas and adipose tissues. Similarity: Belongs to the serpin family. SWISS: O75830 Gene ID: 5276 Database links: Entrez Gene: 5276 Human Entrez Gene: 67931 Mouse Entrez Gene: 171149 Rat Omim: 605587 Human SwissProt: O75830 Human SwissProt: Q9JK88 Mouse Unigene: 445555 Human Unigene: 259446 Mouse Unigene: 54500 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验liteng1117 求助: 构建好了重组腺病毒进行蛋白表达,分子量是14kd,5%的浓缩胶70v,12%,或15%的分离胶110v,跑90min。湿转:100v,90min。封闭用5%的脱脂奶粉,封闭3h(因为背景深,以为封闭久会好些)。一抗1:3000,4度过夜,二抗1:5000,孵育1h,洗膜都是用TBST漂洗15min ×4次。但是显影后背景特别深,虽然可以看见目的条带,但还是有一些非特异性的条带出现,不知道是封闭出问题还是一抗浓度过高。
不能跟上节奏,也可以通过 OneNote 的录音(录像)功能来进行将内容实时地保存到 OneNote 当中。不用担心重要信息的遗漏啦~14. 账号资料云同步只要你记得自己的账号密码,就算是在别的电脑上登录 OneNote,你也可以看到自己账号内所有的笔记资料,不像传统办公软件,所有资料都要保存为本地文件随身携带。尤其是我们一些在工作中常用的资料,记录在 OneNote 就比较方便,只有 OneNote,就不怕忘记带资料这回事。
KSUD14-STS ASSAY FOR TAGGING Lr21
New D14 primers. Lr21L: CGC TTT TAC CGA GAT TGG TC Lr21R: TCT GGT ATC TCA CGA AGC CTT Lr21 : 669 bp lr21 -Fielder: 757 bp lr21 -Wichita: 774 bp Recipe (in 25 µl reaction
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