- ¥1380 - 2200
- LMAI Bio
- LM-6349R
- 进口/国产
- 2025年12月02日
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Cow, Horse,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-6349R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
VGF
- 抗体名：
神经生长因子诱导蛋白抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Cow, Horse,
- 免疫原：
KLH conjugated synthetic peptide derived from human Nerve growth factor inducible:451-550/615
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | VGF |
| 中文名称 | 神经生长因子诱导蛋白抗体 |
| 别 名 | NERP-1; NERP-2; Nerve growth factor inducible; Neuroendocrine regulatory peptide-2; Neurosecretory protein VGF; VGF_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 65kDa |
| 细胞定位 | 细胞浆 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nerve growth factor inducible:451-550/615 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity Function: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. Subcellular Location: Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus. Tissue Specificity: Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. Post-translational modifications: Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure. SWISS: O15240 Gene ID: 7425 Database links: Entrez Gene: 7425 Human Entrez Gene: 381677 Mouse Entrez Gene: 29461 Rat Omim: 602186 Human SwissProt: O15240 Human SwissProt: P20156 Rat Unigene: 587325 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VGF) Polyclonal Antibody, Unconjugated (bs-6349R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
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文献和实验大鼠VGF 神经生长因子诱导蛋白(VGF) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆，细胞上清及相关液体样本中VGF 神经生长因子诱导蛋白(VGF) 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中大鼠 VGF 神经生长因子诱导蛋白 (VGF) 水平。用纯化的大鼠 VGF 神经生长因子诱导蛋白 (VGF) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔
TLQP-21 是一种生物活性肽，来源于神经分泌蛋白VGF的特定区域，按神经生长因子反应基因的编码。TLQP-21调节代谢，增加BM水平，刺激肾 上腺髓质，调节胃部动力功能，阻止体重增加。瑞士Bachem公司生产上市的TLQP-21 酶免检测试剂盒产品采用高特异性抗体，适用于检测人和 动物样品中的TLQP-21。 货号 产品
园lixinwuhan的观点：pc12细胞可以被神经生长因子诱导分化成类神经元细胞，会长出轴突，和神经元细胞的结构和功能类似。未分化的可以用来做NGF的诱导实验等等，已分化的应该可以做神经元细胞相关的检测吧，像免疫组化什么的。培养用1640+10%FCS+5%HS,细胞生长很快，3天左右就要传一代，若要分化的话用NGF诱导分化，具体浓度说法不一，我用100ng/ml诱导成功过，会长出很长的轴突。关于胎牛血清和马血清的作用有人做过相关研究，指出马血清提供了PC12细胞神经样生长突触所需的重要因子，胎牛血清在PC
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