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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 保存条件:
见产品外包装
- 库存:
需确认
- 保质期:
见产品外包装
- 英文名:
ECL BLOCKING AGENT
上海嵘崴达实业有限公司,专业经营生命科学领域的科研试剂、仪器和实验室消耗品,
致力于向国内生命科学研究人员提供合格产品和完善服务。
主要经营的试剂品牌:
Roche试剂,部分常用现货,周三定货,2-3周到货。
GE试剂,耗材,部分常用现货,周二,周五定货,3周左右。
SIGMA试剂,周一,五定货,3周左右到货。
R&D试剂,周一定货,3-4周到货。
THERMO试剂,部分现货,周三定货,2-3周到货。
ABCAM试剂,国内现货三天左右,期货四周左右。
Peprotech试剂,部分常用现货,周三定货,2-3周到货。
罗氏原料,请来电来邮咨询。
OXOID,BD培养基货期八周左右,部分现货。
MCE激动剂,ACROS、ALFA、阿拉丁等生化试剂现货定购。
| RPN2125 | ECL BLOCKING AGENT |
| RPN2134 | ECL WESTERN BLOTTING REAGENTS |
| RPN2209 | ECL WESTERN BLOTTING DETECTION REAGENTS |
| RPN2232 | ECL Prime Western Blotting Detection Reagent |
| RPN2235 | ECL Select WB Detection Reagent |
| RPN2236 | ECL Prime Western Blot Detection Reagent |
| RPN3243 | ECL start WB Detection Reagent - 200ml |
| RPN3244 | ECL start WB Detection Reagent - 400ml |
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文献和实验-T►Add primary antibody diluted into blocking solution. Incubate at room►temp for 1 hour. (Can be left at 4℃ O/N here)►Wash briefly 2X then 3X10 min each with TBS-T►Add secondary HRP conjugated antibody diluted into blocking solution. Incubate at room
Western blotting using ECL reagent
REAGENTSECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples).Hybond ECL nitrocellulose
Blocking of unwanted non-specific staining
7.5, to which 0.01 Tween 20 has been added (TBS-T). 2- Briefly blot the slides without letting them dry and then apply egg white in PBS-BSA-NaN3 as a blocking agent (one egg white in 100 ml PBS + NaN3) (Miller RT et al, Appl Immunohistochemistry 5(1): 63-66
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