- ¥1380 - 2200
- LMAI Bio
- LM-1479R
- 进口/国产
- 2025年07月13日
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1µg/Test （石蜡切片需做抗原修复）
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-1479R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
CD80
- 抗体名：
刺激分子B7-1蛋白抗体
- 宿主：
Rabbit
- 适应物种：
Human
- 免疫原：
KLH conjugated synthetic peptide derived from human CD80:111-265/288 <Extracellular>
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1µg/Test （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | CD80 |
| 中文名称 | 刺激分子B7-1蛋白抗体 |
| 别 名 | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 免疫学 干细胞 淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1µg/Test （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 32kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD80:111-265/288 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. Function: Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Subcellular Location: Membrane. Tissue Specificity: Expressed on activated B-cells, macrophages and dendritic cells. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P33681 Gene ID: 941 Database links: Entrez Gene: 941 Human Entrez Gene: 12519 Mouse Omim: 112203 Human SwissProt: P33681 Human SwissProt: Q00609 Mouse Unigene: 838 Human Unigene: 89474 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Sample:
HL-60(Human) Cell Lysate at 40 ug Primary: Anti-CD80 (bs-1479R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 57 kD 
Blank control: U937(blue).
Primary Antibody: Rabbit Anti-CD80 antibody(bs-1479R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-1479R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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文献和实验CD80 分子 CD80 常用单克隆抗体或代号： B7- 1,BB1 主要表达细胞： Ba,Mac,TStr [B] 分子质量(kDa)和结构： gp60(IgSF) 功 能： 活化B细胞抗原,CD28、CTLA- 4配体,提供T细胞共刺激信号 CD80 Aka B7-1, BB1 Ligand for T cell marker CD28 or CTLA4 (CD152
CD86 分子 CD86 常用单克隆抗体或代号： FUN- 1,BU63,GR65;(B7- 2) 主要表达细胞： Ba,M [B] 分子质量(kDa)和结构： gp80(IgSF) 功 能： CD28、CTLA- 4配体,提供T细胞共刺激信号 CD86 Summary: Costimulates IL-2 production and T cell proliferation
基因与目的抗原基因导人表达载体，可以极大地增强细胞免疫应答。B7-1/B?-2即CD80/CD86为常见的共刺激分子，实验证明将弱免疫原性的流感病毒核蛋白突变株基因与B7-2基因共同导人表达载体后免疫小鼠，可获得显著地针对病毒核蛋白的细胞免疫应答。此外，众多证据表明B7-1/B7-2可以影响免疫反应的类型，两者均能诱导IL-2、IFN-丁和GM-CSF产生；B?-2主要诱导IL-4，结果使免疫途径向Th2发展。 CD40/CD40L协调刺激作用也可调节细胞免疫和体液免疫，调节
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