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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
regulatory protease in the blood coagulation system, is used as a model system to demonstrate a high-throughput procedure to quantitatively characterize substrate sequences and their susceptibilities for enzymatic cleavage. This methodology can be generalized
TUNEL PROCEDURE IN BOVINE EMBRYOS
1:10 poly-l-lysine solution (Sigma P8920) for 2 minutes. Allow the slides to dry. DNase (50 U/ml) - The stock is RQ1-RNA free DNase, Promega cat. # 610A, concentration 1U/µl. Dilute
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