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上海睿星基因技术有限公司从2001年开始为科研人员提供单克隆/多克隆抗体制备技术服务，从2006年起和复旦大学管坤良&熊跃实验室（MCB lab）合作制备乙酰化抗体，磷酸化抗体，甲基化抗体，MCB lab发表文章列表如下。上海睿星基因技术有限公司在修饰抗体制备方面积累了大量的经验，为国内外的科研人员制备了大量的位点特异性修饰抗体。
抗体制备技术服务部分客户单位：复旦大学；浙江大学；清华大学；军事医学科学院；
UCSD；Emory University；Novartis；Wako
ShanghaiGenomics provided Customized antibody production service to lifescience researchers from 2001,we can generate the rabbit polyclonal antibody and mouse monoclonal antibody for customers. 17 years experience of Customized antibody production service and commercial antibody products R&D can make our service and antibody products perfect.
We have 12 years experience of site specific antibody production,eg Actylation, phosphorylation,Methylation.Our company collaborate with MCB lab Fudan University for site specific antibody production from 2006.The MCB lab has published 47 research papers from 2008.
Publication is listed as below,
MCB lab（esdablished in 2006） PI：Kunliang Guan & Yue Xiong
1 .EMBO Rep.
Chen, X-F., Tian, M-X., Sun, R-Q., Zhang, M-L., Zhou, L-S., Jin, L., Chen, L-L., Zhou, W-J., Duan, K-L., Chen, Y-J., Gao, C., Cheng, Z-L., Wang, F., Zhang, J-Y., Sun, Y-P., Yu, H-X., Zhao, Y-Z., Yang, Y., Liu, W-R., Shi, Y-H., Xiong, Y., Guan, K-L., Ye, D.. (2018) SIRT5 inhibits peroxisomal ACOX1 to prevent oxidative damage and is downregulated in liver cancer. EMBO Rep. Feb 28. pii: e45124. doi: 10.15252/embr.201745124.
Antibodies specific to Flag (Shanghai genomic)
prepare the PKM2 K433 acetylation antibody
4. Lin,R., Ren, T., Gao, X., Zhou, X., Li, T., Xiong, Y., Guan, K-L., Lei, Q-Y..(2013) Acetylation Stabilizes ATP-citrate Lyase to Promote Lipid Biosynthesis and Tumor Growth. Molecular Cell 51, 506–518
Antibodies specifically recognizing acetylation at lysines 540, 546, and 554 (Ac[3K]) were prepared commercially by immunizing rabbits at Shanghai Genomics, Inc.
5. Han, X-R., Zha, Z-Y., Yuan, H-X., Feng, X., Xia, Y-K., Lei, Q-Y., Guan, K-L., and Xiong, Y., (2016) KDM2B/FBXL10 targets c-Fos for ubiquitylation and degradation in response to mitogenic stimulation. Oncogene, 35: 4179-4190.
Antibodies and immunological procedures
Antibodies against FLAG (SG4110-16; Shanghai Genomics Technology ), MYC (SG4110-18; Shanghai Genomics Technology)
6. Zhou, L-S., Wang, F., Sun, R-Q., Chen, X-F., Zhang, M-L.,Xu, Q., Wang, Y., Wang, S-W., Xiong, Y., Guan, K-L.,Yang, P-Y., Yu, H-X., Ye, D.,(2016).SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense. EMBO Rep. 17(6):811-22.
Flag (Shanghai Genomics, 4110-20) were purchased commercially.
7. Wang, P., Wu, J., Ma, S-H., Zhang, L., Yao, J., Hoadley, KA., Wilkerson MD., Perou CM., Guan, K-L., Ye, D., Xiong, Y., (2015).Oncometabolite D-2-Hydroxyglutarate Inhibits ALKBH DNA Repair Enzymes and Sensitizes IDH Mutant Cells to Alkylating Agents. Cell Rep. 13(11):2353-61
Antibodies against Flag (ShanghaiGenomics)
Cancer metabolism lab PI：Qunying Lei
10. Wang YP*, Zhou W, Wang J, Huang X, Zuo Y, Wang TS, Gao X, Xu YY, Zou SW, Liu YB, Cheng JK*, Lei QY*. Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer. Molecular Cell 2016, 64: 673–687. Highlights：Cancer discovery
MDH1 R248 Arginine Methylation antibody
11. Li T, Liu M, Feng X, Wang Z, Das I, Xu Y, Zhou X, Sun Y, Guan KL, Xiong Y, Lei QY*. Glyceraldehyde-3-phosphate Dehydrogenase Is Activated by Lysine 254 Acetylation in Response to Glucose Signal. J Biol Chem. 2014, 289(6):3775-3785.
Glyceraldehyde-3-phosphate Dehydrogenase Lysine 254 antibody
南昌大学生科院&医学院 王建斌实验室
12.Tianyu Han1,2, Weihua Zhan1,2, Mingxi Gan1, Fanrong Liu3, Bentong Yu4, Y. Eugene Chin5and Jian-Bin Wang. Phosphorylation of glutaminase by PKCεis essential for itsenzymatic activity and critically contributes to tumorigenesis.Cell Research (2018) 0:1–15
The anti-phospho-serine 314 specificpolyclonal antibody against GAC (GAC-pS314) was made by Shanghai Genomics Inc
徐彦辉 实验室 复旦大学生物医学研究院
13.Xizi Chen, Mengjie Liu, Yuan Tian, Jiabei Li, Yilun Qi, Dan Zhao, Zihan Wu, Min Huang, Catherine C. L. Wong, Hong-Wei Wang, Jiawei Wang, Huirong Yang* & Yanhui Xu*. Cryo-EM structure of human mTOR complex 2. Cell Research. Published online 22 March 2018
Anti-Myc HRP（GNI4310-MC-S, GNI Group）
After 3 years R&D experiments,Our lab developed the best anti-flag monoclonal antibody around the world in 2004.It remains the best anti-flag monoclonal antibody until now. We sold more than 4000ml anti-flag affinity gel around the world in 2017.We have tag series antibody ,internal contral antibody(β-Actin,GAPDH，β-tubulin,α-tubulin) and anti-tag affinity gel (flag HA Myc V5).
We provide excellent customized monoclonl antibody service to customers from 2004，we collaborate with Novartis for Mab in 2007.
抗体制备技术服务部分客户单位：复旦大学；浙江大学；清华大学；军事医学科学院；
UCSD；Emory University；Novartis；Wako
ShanghaiGenomics provided Customized antibody production service to lifescience researchers from 2001,we can generate the rabbit polyclonal antibody and mouse monoclonal antibody for customers. 17 years experience of Customized antibody production service and commercial antibody products R&D can make our service and antibody products perfect.
We have 12 years experience of site specific antibody production,eg Actylation, phosphorylation,Methylation.Our company collaborate with MCB lab Fudan University for site specific antibody production from 2006.The MCB lab has published 47 research papers from 2008.
Publication is listed as below,
MCB lab（esdablished in 2006） PI：Kunliang Guan & Yue Xiong
1 .EMBO Rep.
Chen, X-F., Tian, M-X., Sun, R-Q., Zhang, M-L., Zhou, L-S., Jin, L., Chen, L-L., Zhou, W-J., Duan, K-L., Chen, Y-J., Gao, C., Cheng, Z-L., Wang, F., Zhang, J-Y., Sun, Y-P., Yu, H-X., Zhao, Y-Z., Yang, Y., Liu, W-R., Shi, Y-H., Xiong, Y., Guan, K-L., Ye, D.. (2018) SIRT5 inhibits peroxisomal ACOX1 to prevent oxidative damage and is downregulated in liver cancer. EMBO Rep. Feb 28. pii: e45124. doi: 10.15252/embr.201745124.
Antibodies specific to Flag (Shanghai genomic)
- Li, F-L., Liu, J-P., Bao, R-X., Yan, G., Feng, X., Xu, Y-P., Sun, Y-P., Yan, W., Ling, Z-Q., Xiong, Y., Guan, K-L., and Yuan, H-X. (2018) Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis. Nat Commun, 9:508.
To generate a site-specific antibody to detect the acetylated K472 of PFKFB3 (anti-acK472, 1:500), synthesized peptide SPEPTK(Ac)KPRINS (Shanghai Genomic Inc.) was coupled to KLH as antigen to immunize rabbit. Anti-serum was collected after four doses of immunization.
prepare the PKM2 K433 acetylation antibody
4. Lin,R., Ren, T., Gao, X., Zhou, X., Li, T., Xiong, Y., Guan, K-L., Lei, Q-Y..(2013) Acetylation Stabilizes ATP-citrate Lyase to Promote Lipid Biosynthesis and Tumor Growth. Molecular Cell 51, 506–518
Antibodies specifically recognizing acetylation at lysines 540, 546, and 554 (Ac[3K]) were prepared commercially by immunizing rabbits at Shanghai Genomics, Inc.
5. Han, X-R., Zha, Z-Y., Yuan, H-X., Feng, X., Xia, Y-K., Lei, Q-Y., Guan, K-L., and Xiong, Y., (2016) KDM2B/FBXL10 targets c-Fos for ubiquitylation and degradation in response to mitogenic stimulation. Oncogene, 35: 4179-4190.
Antibodies and immunological procedures
Antibodies against FLAG (SG4110-16; Shanghai Genomics Technology ), MYC (SG4110-18; Shanghai Genomics Technology)
6. Zhou, L-S., Wang, F., Sun, R-Q., Chen, X-F., Zhang, M-L.,Xu, Q., Wang, Y., Wang, S-W., Xiong, Y., Guan, K-L.,Yang, P-Y., Yu, H-X., Ye, D.,(2016).SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense. EMBO Rep. 17(6):811-22.
Flag (Shanghai Genomics, 4110-20) were purchased commercially.
7. Wang, P., Wu, J., Ma, S-H., Zhang, L., Yao, J., Hoadley, KA., Wilkerson MD., Perou CM., Guan, K-L., Ye, D., Xiong, Y., (2015).Oncometabolite D-2-Hydroxyglutarate Inhibits ALKBH DNA Repair Enzymes and Sensitizes IDH Mutant Cells to Alkylating Agents. Cell Rep. 13(11):2353-61
Antibodies against Flag (ShanghaiGenomics)
- Lv, X-B., Liu, C-Y., Wang, Z., Sun, Y-P., Xiong, Y., Lei, Q-Y., Guan, K-L.,(2015) PARD3 Induces TAZ Activation and Cell Growth by Promoting LATS1 and PP1 Interaction. EMBO Rep. 16(8):975-85.
- Wang, S-W., Jiang, B-W., Zhang, T-F., Liu, L-X., Wang, Y., Wang, Y-P., Chen, X-F., Lin, H-P., Zhou, L-S., Xia, Y-K., Chen, L-L., Yang, C., Xiong, Y., Ye, D., Guan, K-L.(2015) Insulin and mTOR Pathway Regulate HDAC3-Mediated Deacetylation and Activation of PGK1. PLoS Biol.13(9):e1002243. doi: 10.1371
Cancer metabolism lab PI：Qunying Lei
10. Wang YP*, Zhou W, Wang J, Huang X, Zuo Y, Wang TS, Gao X, Xu YY, Zou SW, Liu YB, Cheng JK*, Lei QY*. Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer. Molecular Cell 2016, 64: 673–687. Highlights：Cancer discovery
MDH1 R248 Arginine Methylation antibody
11. Li T, Liu M, Feng X, Wang Z, Das I, Xu Y, Zhou X, Sun Y, Guan KL, Xiong Y, Lei QY*. Glyceraldehyde-3-phosphate Dehydrogenase Is Activated by Lysine 254 Acetylation in Response to Glucose Signal. J Biol Chem. 2014, 289(6):3775-3785.
Glyceraldehyde-3-phosphate Dehydrogenase Lysine 254 antibody
南昌大学生科院&医学院 王建斌实验室
12.Tianyu Han1,2, Weihua Zhan1,2, Mingxi Gan1, Fanrong Liu3, Bentong Yu4, Y. Eugene Chin5and Jian-Bin Wang. Phosphorylation of glutaminase by PKCεis essential for itsenzymatic activity and critically contributes to tumorigenesis.Cell Research (2018) 0:1–15
The anti-phospho-serine 314 specificpolyclonal antibody against GAC (GAC-pS314) was made by Shanghai Genomics Inc
徐彦辉 实验室 复旦大学生物医学研究院
13.Xizi Chen, Mengjie Liu, Yuan Tian, Jiabei Li, Yilun Qi, Dan Zhao, Zihan Wu, Min Huang, Catherine C. L. Wong, Hong-Wei Wang, Jiawei Wang, Huirong Yang* & Yanhui Xu*. Cryo-EM structure of human mTOR complex 2. Cell Research. Published online 22 March 2018
Anti-Myc HRP（GNI4310-MC-S, GNI Group）
After 3 years R&D experiments,Our lab developed the best anti-flag monoclonal antibody around the world in 2004.It remains the best anti-flag monoclonal antibody until now. We sold more than 4000ml anti-flag affinity gel around the world in 2017.We have tag series antibody ,internal contral antibody(β-Actin,GAPDH，β-tubulin,α-tubulin) and anti-tag affinity gel (flag HA Myc V5).
We provide excellent customized monoclonl antibody service to customers from 2004，we collaborate with Novartis for Mab in 2007.
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就在有丝分裂、细胞死亡、DNA 损伤修复、DNA 复制和重组过程中发挥着直接的作用。 组蛋白翻译后修饰多发生在组蛋白的 N-端尾部，包括甲基化、乙酰化、磷酸化、ADP-核糖基化、泛素化和小分子泛素化修饰，这些修饰有助于其他蛋白质与 DNA 的结合，从而产生协同或者拮抗作用来调控基因转录。例如，乙酰化使组蛋白尾部正电荷减少，从而削弱了与带负电荷 DNA 骨架的作用，而促进染色质呈开放状态, 甲基化激活或抑制基因功能主要依赖于修饰的位点，主要与赖氨酸残基的单甲基化、双甲基化或三甲基化有关。 组蛋白修饰最基本
蛋白质翻译后修饰 (Protein translational modifications，PTMs) 通过功能基团或蛋白质的共价添加、调节亚基的蛋白水解切割或整个蛋白质的降解来增加蛋白质组的功能多样性。这些修饰包括磷酸化、糖基化、泛素化、亚硝基化、甲基化、乙酰化、脂质化和蛋白水解，几乎影响正常细胞生物学和发病机制的所有方面。因此，识别和理解 PTM 在细胞生物学和疾病治疗和预防的研究中至关重要。 看到一篇 Thermo Fisher的文章，关于翻译后修饰Post
【求助】P53的翻译后修饰都有哪些？ 怎样验证其修饰后与靶标启动子的结合活性的变化？
蛋白质功能的一个主要机制，p53在多个位点上可被磷酸化、顺-反异构化、乙酰化、泛素化、甲基化、糖基化等修饰，从而显示其生物学重要性。这种显示细胞或组织特异性并依赖细胞周期位置的多重修饰，是一种复杂的调节方式，随着细胞对DNA损伤、增殖、老化等产生的细胞信号的反应而发生波动。 磷酸化修饰 P53的磷酸化在多数情况下与蛋白质的稳定性有关。p53 N-末端的三个位点Ser15、Thr18、Ser20磷酸化后，使p53和其主要的负性调节因子MDM2之间的相互作用消失，而和乙酰
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