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- 12571063
- 美国
- 2025年10月15日
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- 文献和实验
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- 库存：
99
- 保质期：
已收到实际货物为准
- 供应商：
北京诺博莱德科技有限公司
- 保存条件：
已收到实际货物为准
货号： 12571063
规格
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Liquid |
| HEPES Buffer: | No HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Volume: | 500 ml |
| Glucose: | Low Glucose |
| Product Size: | 500 mL |
| Serum Supplementation: | Standard Serum Supplementation |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
描述
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. Life Technologies offers a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
| With |
| • Ribonucleosides |
| • Deoxyribonucleosides |
| • Phenol Red |
| • L-glutamine |
The complete formulation is available.
Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® MEM α product made in our Scotland facility (22571-020). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
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文献和实验相关专题 MEM细胞培养基 细胞培养是实验室里最常见和最基本的实验了，但却不是最简单的。别小看细胞培养，这里可蕴含着大学问。有时候细胞状态不好，转染 、药筛这些实验根本就没办法做。影响细胞培养的因素很多，让我们先从培养基和血清说起。 经典的培养基有很多种，Invitrogen (GIBCO)、Thermo Fisher(HyClone)、Sigma等公司都可以提供。其中DMEM、RPMI 1640、MEM、DMEM
% FBS (ES-020-B), 1x 谷氨酰胺 (TMS-002), and 1x P/S 双抗(P4333). 在使用前，向培养基中添加5 ng/ml FGF (GF003AF) (培养基中含有 FGF后可以在4°C 最多存贮一周, 避免反复冷热循环). 3D MSC扩增培养基: 向α-MEM (M4526)中添加10% FBS (ES-020-B), 1x 谷氨酰胺 (TMS-002), and 1x P/S 双抗(P4333). 在使用前，向培养基中添加50 ng/ml FGF (GF
相关专题 细胞培养基的配制 细胞培养专题 经典的培养基有很多种，Invitrogen (GIBCO)、Thermo Fisher(HyClone)、Sigma等公司都可以提供。其中DMEM 、RPMI 1640、MEM、DMEM /F12都是应用最广泛的培养基。其他如M199、IMDM、L15培养基等也用于某些细胞的培养。 ◆ MEM是由Eagle’s基础培养基(BME)发展而来的，其中增加了组分的范围及浓度
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