CRB2蛋白抗体

CRB2蛋白抗体

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  • ¥1380 - 2200
  • LMAI Bio
  • LM-14046R
  • 进口/国产
  • 2025年07月14日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, 
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    • 详细信息
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-14046R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      CRB2

    • 抗体名

      CRB2蛋白抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, 

    • 免疫原

      KLH conjugated synthetic peptide derived from human CRB2:701-800/1285 <Extracellular>

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      100ul  200ul

    CRB2蛋白抗体
    英文名称 CRB2
    中文名称 CRB2蛋白抗体
    别    名 Crb2; CRUM2_HUMAN; Crumbs homolog 2 (Drosophila); Crumbs homolog 2; Crumbs-like protein 2; RP11-230L22.2.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 细胞生物  免疫学  神经生物学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 100, 123, 131kDa
    细胞定位 细胞浆 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CRB2:701-800/1285 <Extracellular>
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    CRB2 is a 1,285 amino acid protein that contains three laminin G-like domains and 15 EGF-like domains and exists as multiple alternatively spliced isoforms that are either secreted or membrane bound. Expressed in kidney and retina, as well as in fetal eye and brain, CRB2 is thought to play a role in the morphogenesis of polarized cells and may be involved in DNA repair. Defects in the gene encoding CRB2 are associated with the pathogenesis of autosomal recessive retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA), the former of which refers to a group of diseases that lead to the degeneration of retinal photoreceptor cells, ultimately resulting in a loss of vision. The gene encoding CRB2 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome.

    Function:
    May play a role in polarized cells morphogenesis.

    Subcellular Location:
    Secreted and Cell membrane.

    Tissue Specificity:
    Expressed in retina, fetal eye and brain. Also expressed in kidney, ARPE-19 and RPE/choroid cell lines, and at low levels in lung, placenta, and heart.

    Similarity:
    Belongs to the Crumbs protein family.
    Contains 15 EGF-like domains.
    Contains 3 laminin G-like domains.

    SWISS:
    Q5IJ48

    Gene ID:
    286204

    Database links:

    Entrez Gene: 286204 Human

    Entrez Gene: 241324 Mouse

    Entrez Gene: 366031 Rat

    Omim: 609720 Human

    SwissProt: Q5IJ48 Human

    SwissProt: Q80YA8 Mouse

    Unigene: 710092 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 CRB2蛋白抗体
    Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated(bs-14046R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    CRB2蛋白抗体
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRB2) Polyclonal Antibody, Unconjugated (bs-14046R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    CRB2蛋白抗体
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated(bs-14046R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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