- ¥1380 - 2200
- 康朗生物
- kl-6589R
- 中国/美国/德国
- 2025年11月28日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
- Rabbit
- Human, Mouse, Rat,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-6589R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
BCAT2 antibody
- 抗体名：
支链氨基酸转氨酶2抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat,
- 免疫原：
KLH conjugated synthetic peptide derived from human BCAT2/BCAM:101-200/392
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul 200ul
| 中文名称 | 支链氨基酸转氨酶2抗体 |
| 别 名 | BCAM; BCAT 2; BCATM; BCT2; Branched chain aminotransferase 2, mitochondrial; ECA40; Placental protein 18; PP18. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 41kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BCAT2/BCAM:101-200/392 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Class-IV pyridoxal-phosphate-dependent aminotransferase family members ECA39 and BCAT2 are both enzymes that catalyze the first reaction in the catabolism of the essential branched chain amino acids valine, leucine and isoleucine. ECA39, also known as BCAT1 (branched-chain-amino-acid aminotransferase 1, cytosolic) is localized to the cytoplasm where it forms a homodimer. ECA39 is expressed in the brain and kidney during embryogenesis and is overexpressed in c-Myc induced tumors. BCAT2 (branched-chain-amino-acid aminotransferase 2, mitochondrial), also known as placental protein 18 (PP18), is expressed as two isoforms produced by alternative splicing. The first isoform of BCAT2, designated BCAT2A, is expressed in the mitochondrion, while the second isoform, designated BCAT2B, is expressed in the cytoplasm. Ubiquitously expressed, BCAT2 is also thought to act as a transporter of branched chain α-keto acids. Function: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Subunit: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Subcellular Location: Cytoplasm and Mitochondrion. Tissue Specificity: Ubiquitous. Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. SWISS: O15382 Gene ID: 587 Database links: Entrez Gene: 587 Human Entrez Gene: 12036 Mouse Omim: 113530 Human SwissProt: O15382 Human SwissProt: O35855 Mouse Unigene: 512670 Human Unigene: 24210 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-BCAT2 (bs-6589R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD  Sample: Kidney (Mouse) Tissue Lysate at 30 ug Primary: Anti-BCAT2(bs-6589R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BCAT2/BCAM Polyclonal Antibody, Unconjugated(bs-6589R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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