phospho-IRS1 (Ser616)磷酸化胰岛素受体底

物1抗体

价格¥1580

品牌LMAI Bio
货号LM-12710R
产地进口/国产
更新日期2025年07月16日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
宿主Rabbit
适应物种Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,

上海联迈生物工程有限公司

10 年钻石会员

相关产品推荐更多 >

万千商家帮你免费找货

0 人在求购买到急需产品

详细信息
文献和实验
技术资料

供应商：
上海联迈生物工程有限公司
库存：
大量
目录编号：
LM-12710R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
1年
抗体英文名：
phospho-IRS1 (Ser616)
抗体名：
磷酸化胰岛素受体底物1抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
免疫原：
KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser616:PM(p-S)PG
亚型：
IgG
形态：
Lyophilized or Liquid
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
浓度：
1mg/ml
保存条件：
Store at -20 °C
规格：
100ul

phospho-IRS1 (Ser616)磷酸化胰岛素受体底物1抗体

英文名称	phospho-IRS1 (Ser616)
中文名称	磷酸化胰岛素受体底物1抗体
别名	IRS1 (phospho S616); p-IRS1 (phospho S616); HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS 1; IRS-1; IRS1; IRS1_HUMAN; OTTHUMP00000164234
规格价格	100ul/1580元购买大包装/询价
说明书	100ul
产品类型	磷酸化抗体
研究领域	细胞生物免疫学神经生物学细胞凋亡糖尿病细胞类型标志物
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	131kDa
细胞定位	细胞核细胞浆细胞膜
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser616:PM(p-S)PG
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009] Function: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Subunit: Interacts with UBTF and PIK3CA (By similarity). Interacts (via phosphorylated YXXM motifs) with PIK3R1 (By similarity). Interacts with ROCK1 and FER (By similarity). Interacts (via PH domain) with PHIP (By similarity). Interacts with GRB2 (By similarity). Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK. Interacts with EIF2AK2/PKR (By similarity). Post-translational modifications: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding. DISEASE: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Similarity: Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: P35568 Gene ID: 3667 Database links: Entrez Gene: 3667 Human Entrez Gene: 16367 Mouse Entrez Gene: 25467 Rat Omim: 147545 Human SwissProt: P35568 Human SwissProt: P35569 Mouse SwissProt: P35570 Rat Unigene: 471508 Human Unigene: 4952 Mouse Unigene: 10476 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD Sample: MCF-7 Cell (Human) Lysate at 40 ug Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD Sample: MCF-7 Cell (Human) Lysate at 40 ug Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD Sample: MG63(Human) Cell Lysate at 30 ug Primary: Anti-phospho-IRS1 (Ser616) (bs-12710R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-IRS1 (Ser616)) Polyclonal Antibody, Unconjugated (bs-12710R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Ser616) antibody (bs-12710R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.