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CD90抗体

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  • ¥780 - 2200
  • LMAI Bio
  • LM-0778R
  • 进口/国产
  • 2025年07月07日
  • WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Dog, Pig, Horse, Sheep, 
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-0778R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      CD90

    • 抗体名

      CD90抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Dog, Pig, Horse, Sheep, 

    • 免疫原

      KLH conjugated synthetic peptide derived from rat Thy-1 (52-100aa):31-120/161 <Extracellular>

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      50ul  100ul  200ul

    CD90抗体
    英文名称 CD90
    中文名称 CD90抗体
    别    名 CD90 / Thy1; CD7; CD90; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN.  
    产品细节图片1 Specific References  (12)     |     bs-0778R has been referenced in 12 publications.
    [IF=6.72] Chen, Guobao, et al. "3D Scaffolds with Different Stiffness but Same Microstructure for Bone Tissue Engineering." ACS Applied Materials & Interfaces (2015).  IHC-F ;  Rat.  
    PubMed:26151287
    [IF=1.52] Tepekoy, Filiz, et al. "CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development." Reproductive Biology(2015).  IHC-P ;  Mouse.  
    PubMed:26679159
    [IF=3.36] Long, Q., et al. "Bone marrow mesenchymal stem cell transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." Neuroscience (2015).  Rat.  
    PubMed:26545982
    [IF=1.80] Vansandt, Lindsey M., et al. "Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia." Theriogenology(2016).  IHC-P ;  Other Species.  
    PubMed:27129396
    [IF=1.43] Zeng, Biao, et al. "Increased expression of importin13 in endometriosis and endometrial carcinoma." Medical Science Monitor 18.6 (2012): CR361-CR367.  IHC-P ;  Human.  
    PubMed:22648251
    [IF=1.06] Scott, Erin M., et al. "Early histopathologic changes in the retina and optic nerve in canine primary angle‐closure glaucoma." Veterinary ophthalmology16.s1 (2013): 79-86.  IHC-P ;  Dog.  
    PubMed:23826772
    [IF=0.00] Gstraunthaler, Gerhard, et al. "Human platelet lysates successfully replace fetal bovine serum in adipose-derived adult stem cell culture." Journal of Advanced Biotechnology and Bioengineering 2.1 (2014): 1-11.  IF(ICC) ;  Human.  
    PubMed:0
    [IF=0.52] Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.  IF(ICC) ;  Rat.  
    PubMed:0
    [IF=1.92] Ma, Caiyun, et al. "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." Cryobiology (2017).  FCM ;  Sheep.  
    PubMed:28284665
    [IF=2.94] Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro." Brain Research Bulletin (2013).  FCM ;  Rat.  
    PubMed:24144723
    [IF=2.88] Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).  Rat.  
    PubMed:23928226
    [IF=2.26] Yu, Xian-huan, et al. "Clinicopathological characteristics of 20 cases of hepatocellular carcinoma with bile duct tumor thrombi." Digestive diseases and sciences 56.1 (2011): 252-259.  IHC-P ;  Human.  
    PubMed:20437099
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 细胞生物  免疫学  神经生物学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, Sheep, 
    产品应用 WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 12kDa
    细胞定位 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from rat Thy-1 (52-100aa):31-120/161 <Extracellular>
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.

    Function:
    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

    Subcellular Location:
    Cell membrane; Lipid-anchor, GPI-anchor.

    Tissue Specificity:
    Abundant in lymphoid tissues.

    Post-translational modifications:
    Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.

    Similarity:
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    SWISS:
    P01830

    Gene ID:
    24832

    Database links:

    Entrez Gene: 7070 Human

    Entrez Gene: 21838 Mouse

    Entrez Gene: 24832 Rat

    Omim: 188230 Human

    SwissProt: P04216 Human

    SwissProt: P01831 Mouse

    SwissProt: P01830 Rat

    Unigene: 644697 Human

    Unigene: 3951 Mouse

    Unigene: 108198 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片2
    Sample: 
    Brain (Mouse) Lysate at 40 ug
    Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 12 kD
    Observed band size: 32 kD

    产品细节图片3
    Sample: 
    Brain (Mouse) Lysate at 40 ug
    Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 12 kD
    Observed band size: 32 kD

    产品细节图片4
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-Thy-1/CD90/ Thy1.1 Polyclonal Antibody, Unconjugated(bs-0778R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    产品细节图片5
    产品细节图片6
    Blank control: U937(blue). 
    Primary Antibody: Rabbit Anti-CD90 antibody(bs-0778R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; 
    Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    Protocol
    The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-0778R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
    产品细节图片7
    Blank control (blue line): U251 (blue). 
    Primary Antibody (green line): Rabbit Anti- CD90 antibody (bs-0778R) 
    Dilution: 1μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
    Dilution: 1μg /test. 
    Protocol
    The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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    图标文献和实验
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      CD90 分子 CD90 常用单克隆抗体或代号: 5E10;(Thy1.23) 主要表达细胞: Pre- B,Pro- Hem,大脑[AS] 分子质量(kDa)和结构: gp 25- 35 (GPI连接)(IgSF) 功 能: T细胞活化、识别、粘附 CD90 Summary: May mediate differentiation of hemaoietic stem cells and synaptogenesis

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