- ¥780 - 2200
- LMAI Bio
- LM-1270R
- 进口/国产
- 2025年07月12日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Cow, Horse,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-1270R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
CK16
- 抗体名：
细胞角蛋白16抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Cow, Horse,
- 免疫原：
KLH conjugated synthetic peptide derived from human CK16:371-473/473
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | CK16 |
| 中文名称 | 细胞角蛋白16抗体 |
| 别 名 | Cytokeratin 16; CK 16; Focal non epidermolytic palmoplantar keratoderma; K 16; K16; K1CP; CK-16; CK16; Cytokeratin-16; FNEPPK; K1C16_HUMAN; Keratin; Keratin-16; type I cytoskeletal 16; Keratin 16; Keratin type I cytoskeletal 16; Keratin16; KRT 16; KRT16; KRT16A; NEPPK. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 免疫学 信号转导 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 51kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CK16:371-473/473 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]. Subunit: Heterodimer of a type I and a type II keratin. KRT16 associates with KRT6 isomers. Interacts with TCHP. Interacts with TRADD (By similarity). Tissue Specificity: Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts. DISEASE: Pachyonychia congenita 1 (PC1) [MIM:167200]: An autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present. Note=The disease is caused by mutations affecting the gene represented in this entry. Keratoderma, palmoplantar, non-epidermolytic, focal (FNEPPK) [MIM:613000]: A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present. Note=The disease is caused by mutations affecting the gene represented in this entry. Unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]: UPVN is characterized by a localized epidermolytic hyperkeratosis in parts of the right palm and the right sole, following the lines of Blaschko. Note=The disease is caused by mutations affecting the gene represented in this entry. Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris. Similarity: Belongs to the intermediate filament family. SWISS: P08779 Gene ID: 3868 Database links: Entrez Gene: 3868 Human Omim: 148067 Human SwissProt: P08779 Human Unigene: 655160 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Placenta(Mouse) Lysate at 30 ug Testis (Mouse) Lysate at 30 ug Primary:Anti-CK16 (bs-1270R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 51 kD Observed band size: 55 kD  Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK16) Polyclonal Antibody, Unconjugated (bs-1270R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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文献和实验利用 Millicell 培养小室培养皮肤及肺类器官的实验方案
formation and lung inflammation in cystic fibrosis through ROS-mediated autophagy inhibition. Nat Cell Biol. 12(9):863-75. 细胞角蛋白18 泛细胞角蛋白 ZO-1紧密连接 CFTR (WT) 图3.16HBE14o细胞系的抗体鉴定。从左至右:16HBE14o-细胞表达肺上皮关键标志物细胞角蛋白18，泛细胞角蛋白(绿色，MAB3412)， TJ蛋白ZO-1证实形成紧密连接(红色，AB
-GFP感染分化第 13 天的细胞群，在第 16 天对细胞群通过免疫荧光显微术（左图）以及 GFP 荧光分析－活化细胞分选（FACS）分析（右图）。 （C）通过定量逆转录（qRT）-PCR分析，在分选之前和之后（分别于分化的第13天和第16天）分别测定肝祖细胞中的细胞角蛋白（CK）19转录物水平。 （D）将人胚胎干细胞（hESC）衍生的肝祖细胞的相位对比图像铺在I型胶原上，并在分选后培养48小时（放大倍数×100）。在 1 型胶原上培养 48 小时后，用甲胎蛋白（AFP）（绿色）和 CK19（红色
能和 FcγR Ⅲ/Ⅱ结合，封闭非特异性染色，使阴性细胞的背景荧光降至未标记细胞的水平。加入 0.5-1μg 纯的抗小鼠 CD16/32 单克隆抗体，室温孵育 10min。 对于人和大鼠，可直接使用过量的与荧光抗体相同来源和亚型的纯化 Ig 或者与目标种属相同来源血清进行阻断，或者用商业化的 Fc 受体阻断剂。 五、细胞表面抗体孵育 根据实验设计，除空白对照外，对应的单染管、全染管加入相应的抗体 5μL，混匀，4℃ 避光孵育 30min。 六、固定破膜 按照说明书稀释固定破膜液。 每管加入
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