BDNF antibody

价格¥780 - 2200

品牌康朗生物
货号
kl-4989R
产地中国/美国/德国
更新日期2025年11月25日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:200-800
宿主Rabbit
适应物种Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,

上海康朗生物科技有限公司

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详细信息
技术资料

供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-4989R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
BDNF antibody
抗体名：
脑源神经营养因子抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
免疫原：
KLH conjugated synthetic peptide derived from human BDNF:151-247/247
亚型：
IgG
形态：
冻干粉或液体
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:200-800
浓度：
1mg/ml
保存条件：
-20 °C
规格：
50ul 100ul 200ul

BDNF antibody

中文名称	脑源神经营养因子抗体
别名	Abrineurin; BDNF; BDNF; BDNF_HUMAN; Brain Derived Neurotrophic Factor; Brain-derived neurotrophic factor; MGC34632; MGC34632; Neurotrophin.
	Specific References (1) \| bs-4989R has been referenced in 1 publications. [IF=5.62] Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014). WB ; Rat. PubMed:24780497
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	细胞生物神经生物学信号转导生长因子和激素转录调节因子激酶和磷酸酶细胞因子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:200-800 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	13/27kDa
细胞定位	分泌型蛋白
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human BDNF:151-247/247
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. More recently, three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4), also designated NT-5. These various neurotrophins stimulate the in vitro survival of distinct but partially overlapping populations of neurons. The Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds equally well to both BDNF and NT-4 and to a lesser extent NT-3, while the Trk C receptor only binds NT-3. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Belongs to the NGF-beta family. Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Subunit: Monomers and homodimers. Binds to NTRK2/TRKB. Subcellular Location: Secreted. Tissue Specificity: Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. Post-translational modifications: The propeptide is N-glycosylated and glycosulfated. DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Similarity: Belongs to the NGF-beta family. SWISS: P23560 Gene ID: 627 Database links: Entrez Gene: 627 Human Entrez Gene: 12064 Mouse Entrez Gene: 24225 Rat Omim: 113505 Human SwissProt: P23560 Human SwissProt: P21237 Mouse SwissProt: P23363 Rat Unigene: 502182 Human Unigene: 1442 Mouse Unigene: 11266 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-BDNF (bs-4989R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/27 kD Observed band size: 18/32 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Blank control(blue): HepG2(fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody:Rabbit Anti-BDNF antibody (bs-4989R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.