CD73 antibody

价格¥1380 - 2200

品牌康朗生物
货号kl-4834R
产地中国/美国/德国
更新日期2025年12月23日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500
宿主Rabbit
适应物种Human, Mouse,

上海康朗生物科技有限公司

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供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-4834R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
CD73 antibody
抗体名：
CD73抗体
宿主：
Rabbit
适应物种：
Human, Mouse,
免疫原：
KLH conjugated synthetic peptide derived from human CD73/NT5:151-250/574
亚型：
IgG
形态：
冻干粉或液体
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500
浓度：
1mg/ml
保存条件：
-20 °C
规格：
100ul 200ul

CD73 antibody

中文名称	CD73抗体
别名	NT5; 5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto (CD73); 5'-NT; 5'-nucleotidase; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN antibody eNT; NT antibody NT5; NT5E; NTE; Purine 5 Prime Nucleotidase.
	Specific References (12) \| bs-4834R has been referenced in 12 publications. [IF=1.69] Lu, Taofeng, et al. "Isolation and Characterization of Adipose-derived Mesenchymal Stem Cells (ADSCs) from Cattle." Applied Biochemistry and Biotechnology (2014): 1-10. Rat. PubMed:25086927 [IF=1.69] Gao, Yuhua, et al. "All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells." Applied Biochemistry and Biotechnology (2014): 1-11. Chicken. PubMed:25086923 [IF=1.69] Gao, Yuhua, et al. "All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells." Applied Biochemistry and Biotechnology (2014): 1-11. Chicken. PubMed:25086923 [IF=2.03] Long, Qianfa, et al. "MRI tracking bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy." Neuroscience Letters (2015). Rat. PubMed:26318841 [IF=0.96] Wang, Chenghe, et al. "Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD." Official Journal of the Brazilian Society of Urology 41.4 (2015): 764-772. Rat. PubMed:26401871 [IF=3.36] Long, Q., et al. "Bone marrow mesenchymal stem cell transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." Neuroscience (2015). Rat. PubMed:26545982 [IF=1.28] Chen, Jia, et al. "Biological characterization of metanephric mesenchymal stem cells from the Beijing duck." Experimental and Therapeutic Medicine 11.2 (2016): 439-447. IF(ICC) ; Others. PubMed:26893628 [IF=3.78] Lin, Hsing-Yi, et al. "Isolation and characterization of multipotent mesenchymal stem cells adhering to adipocytes in canine bone marrow." Stem Cells and Development ja (2016). FCM ; Dog. PubMed:27937753 [IF=1.92] Ma, Caiyun, et al. "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." Cryobiology (2017). FCM ; Sheep. PubMed:28284665 [IF=2.94] Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro." Brain Research Bulletin (2013). FCM ; Rat. PubMed:24144723 [IF=2.88] Gao, Yuhua, et al. "Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells." BioMed Research International 2013 (2013). Chicken. PubMed:23984389 [IF=2.88] Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013). Rat. PubMed:23928226
规格价格	100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	100ul 200ul
研究领域	心血管细胞生物免疫学细胞粘附分子细胞表面分子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	63kDa
细胞定位	细胞膜
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human CD73/NT5:151-250/574
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: CD73 is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Subcellular Location: Cell membrane. DISEASE: Defects in NT5E are the cause of calcification of joints and arteries (CAJA). A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. Similarity: Belongs to the 5'-nucleotidase family. SWISS: P21589 Gene ID: 4907 Database links: Entrez Gene: 4907 Human Omim: 129190 Human SwissProt: P21589 Human Unigene: 153952 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: Kidney (mouse)l Lysate at 40 ug Primary: Anti- CD73 (bs-4834R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 65 kD Sample: HepG2 (human)Cell Lysate at 40 ug Primary: Anti- CD73 (bs-4834R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD Blank control:A549. Primary Antibody (green line): Rabbit Anti-CD73 antibody (bs-4834R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.. Acquisition of 20,000 events was performed. Blank control (blue line): Raji (blue). Primary Antibody (green line): Rabbit Anti- Phospho-c-Fos (Ser32) antibody (bs-4834R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol